We narrowed to 29,671 results for: des.2

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CoV2-N-P80R

Plasmid

#177947

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P80R) (N )

Expression

Mammalian

Mutation

P80R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-S235F

Plasmid

#177956

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (S235F) (N )

Expression

Mammalian

Mutation

S235F

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBeast-pT7-lox

Plasmid

#190059

Purpose

Express the bacterial lactate oxidase enzyme under the control of the strong constitutif phage promoter T7

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

lox

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pT7-codA

Plasmid

#190058

Purpose

Express the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

codA

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pYjjZ-RBS- sfGFP

Plasmid

#190054

Purpose

GFP containing reporter plasmid expressing it under the control of the reportedely OxyR/H2O2 inducible pYjjZ promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pKatG-RBS- sfGFP

Plasmid

#190053

Purpose

GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pKatG promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBeast-pOxyS-RBS- sfGFP

Plasmid

#190052

Purpose

GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pOxyS promoter

Depositor

Jerome Bonnet

Article

Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-F629V_A

Plasmid

#145898

Purpose

Insect Expression of DmAGO1-F629V

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

DmAGO1-F629V (AGO1 Fly)

Expression

Insect

Mutation

V315A, F629V, H913R - variation with 2 non silent…

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E K91N

Plasmid

#110121

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

K91N mutation

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E D193E

Plasmid

#110120

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

D193E mutation

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 T18N

Plasmid

#47897

Purpose

Expresses myc tagged Miro2 T18N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 T18N (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

T18N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 A13V

Plasmid

#47896

Purpose

Expresses myc tagged Miro2 A13V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 A13V (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

A13V, constitutively active mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 E208K/E328K

Plasmid

#47900

Purpose

Expresses myc tagged Miro2 V208K/E328K mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 E208K/E328K (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

E208K/E328K, abolishes calcium binding

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 R425V

Plasmid

#47898

Purpose

Expresses myc tagged Miro2 R425V constitutively active mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 R425V (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

R425V, constitutively active mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-myc-Miro2 S430N

Plasmid

#47899

Purpose

Expresses myc tagged Miro2 S430N dominant negative mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 S430N (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

S430N, dominant negative mutant

Promoter

CMV

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pCAGGS-SARS2-S-D614 (C-flag)

Plasmid

#156420

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-G614 (C-flag)

Plasmid

#156421

Purpose

Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Mutation

D614G

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-D614 (NC-flag)

Plasmid

#156418

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-S (Wuhan variant)

Plasmid

#169846

Purpose

Mammalian expression plasmid for S of SARS-CoV-2 (Wuhan variant) with N-terminal c-myc tag

Depositor

Erik Procko

Article

Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

Insert

Protein S

Tags

HA leader and c-myc tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-G614 (NC-flag)

Plasmid

#156419

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Hyeryun Choe, Michael Farzan

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Tags

Flag

Expression

Mammalian

Mutation

D614G

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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