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pmEGFP_a_tubulin_C1

Plasmid

#21039

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmEGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pCRU5HT1-inLuc

Plasmid

#58954

Purpose

HTLV-1 transfer vector that encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is transduced only after the completion of viral cycle replication.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Retroviral

Promoter

CMV/R/U5

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pEB2-mKate2

Plasmid

#104009

Purpose

Plasmid encoding mKate2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mKate2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, K42R, V45A, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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VPS13D^EGFP

Plasmid

#174109

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Tags

EGFP

Expression

Mammalian

Mutation

With an internal EGFP tag in aminoacid position 1…

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nmdCas9-3XmCherry

Plasmid

#64110

Purpose

Expression of Nm dCas9-3XmCherry in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-M13-TEV-C-P2A-TdTomato

Plasmid

#92391

Purpose

produces AAV expressing Cal-light M13-TevC component and TdTomato

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

M13-TEV-C-P2A-TdTomato

Use

AAV and Synthetic Biology

Tags

TdTomato

Expression

Mammalian

Promoter

human synapsin

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pM2s2AsG

Plasmid

#137924

Purpose

E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

Superfolder GFP

Use

Synthetic Biology; For use with e. coli nissle in…

Expression

Bacterial

Promoter

J23101

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE60

Plasmid

#90405

Purpose

STAT5 - Stat5 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT5

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1beta AS4(-)

Plasmid

#59409

Purpose

Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Nrxn1beta AS4(-) (Nrxn1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAG261-His-greenFAST

Plasmid

#160349

Purpose

Expresses His-tagged greenFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-greenFAST

Expression

Bacterial

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-BirA-eCPX-AP

Plasmid

#121907

Purpose

Expresses BirA and eCPX-AP

Depositor

Per Svenningsen

Article

Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

Inserts

BirA (birA Synthetic)
Enhanced Circularly Permuted Outer Membrane Protein X

Tags

6xHis

Expression

Bacterial

Promoter

T7 and pBAD promoter

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE52

Plasmid

#90397

Purpose

STAT1 - STAT1 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT1

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pPROEX Aqua

Plasmid

#42889

Depositor

Fabienne Merola

Article

Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

Insert

Aquamarine

Tags

6xHis and TEV cleavage

Expression

Bacterial

Mutation

T65S and H148G

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-R-GFP

Plasmid

#11953

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-R-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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Human Bcl-2/pcDNA3

Plasmid

#19279

Depositor

Philip Leder

Article

Fantin et al Cancer Res. 2004 Jan 1. 64(1):329-36.

Insert

human Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pSGNluc

Plasmid

#186705

Purpose

A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

Depositor

Baubak Bajoghli, Maria Leptin

Article

Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

Inserts

GFP
luciferase

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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hCas9_D10A

Plasmid

#41816

Purpose

Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9_D10A

Use

CRISPR

Expression

Mammalian

Mutation

human codon-optimized, D10A nickase

Promoter

CMV

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE26

Plasmid

#90368

Purpose

C/EBPb - CEBPB gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

CEBPb

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_479

Plasmid

#179099

Purpose

BE Cas expression

Depositor

John Doench, David Root

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

ABE8e (SpG)

Use

CRISPR and Lentiviral

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-SENP3

Plasmid

#34554

Depositor

Mary Dasso

Article

Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

Insert

SENP3 (SENP3 Human)

Tags

EGFP

Promoter

CMV

Available Since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

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