We narrowed to 6,522 results for: sas

1. pICOZ-MAG-M235Q

   Plasmid

   #218299

   Purpose

   Mammalian expression vector for expression of MAG transposase mutant

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   M235Q mutant of MAG transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hAT-7_PM

   Plasmid

   #217147

   Purpose

   Mammalian expression vector for expression of hAT-7_PM transposase (CMV promoter)

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   hAT-7_PM transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-HAT1_AG

   Plasmid

   #217113

   Purpose

   Mammalian expression vector for expression of HAT1_AG transposase (CMV promoter)

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   HAT1_AG transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. GPR113-Tango

   Plasmid

   #66304

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR113 (ADGRF3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. GPR3-Tango

   Plasmid

   #66350

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR3 (GPR3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS_4xMma PylT(UUA)_FLAG-AcKRS

   Plasmid

   #140010

   Purpose

   Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   AcKRS-3 (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L301M, L305I, Y306F, L309A, C348F

   Promoter

   EF1

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Blast PhLP1-myc-6xHis

   Plasmid

   #218178

   Purpose

   Used to make a lenti virus for stable expression of phosducin-like protein 1 in mammalian cells

   Depositor

   Barry Willardson

   Article

   Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

   Insert

   Phosducin like protein 1 (PDCL Human)
   

   Use

   Lentiviral

   Tags

   6x His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFL His-mEos3.2-I3

   Plasmid

   #113516

   Purpose

   Expression of His-Eos-I3 in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Use

   Tags

   6*His-mEos3.2

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. GPR25-Tango

   Plasmid

   #66347

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR25 (GPR25 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. GPR32-Tango

    Plasmid

    #66352

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR32 (GPR32 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-6P-1 p37

    Plasmid

    #113500

    Purpose

    Bacterial expression of GST-tagged p37

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRL838

    Plasmid

    #70680

    Purpose

    This vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.

    Depositor

    Peter Wolk

    Article

    Kaneko et al DNA Res. 2001 Oct 31;8(5):205-13; 227-53

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. GPR31-Tango

    Plasmid

    #66351

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR31 (GPR31 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pAS_4xG1 PylT FLAG-G1 PylRS

    Plasmid

    #154768

    Purpose

    plasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_4xEcoLeuT(CUA)_EF1_AnapRS

    Plasmid

    #174894

    Purpose

    AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    AnapRS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2/CMV-eGFP.PB/PTK

    Plasmid

    #170540

    Purpose

    Expresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restored

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    Delta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS_4xMx PylT C41CA_FLAG_Mx PylRS

    Plasmid

    #140013

    Purpose

    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST

    Plasmid

    #182547

    Purpose

    Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificity

    Depositor

    Robert de Vries

    Article

    Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

    Insert

    H5_Vietnam_1203_04_Y161A
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    Y161A mutant that confers exclusive NeuAc to NeuG…

    Promoter

    CMV

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

    Plasmid

    #140014

    Purpose

    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y126A, Y197F

    Promoter

    EF1

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUPD2 PhiC31 integrase (GB1496)

    Plasmid

    #160578

    Purpose

    Streptomyces phage PhiC31 integrase, complete CDS

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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