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Showing: 5361 - 5380 of 7681 results

  6. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72949-plasmid-map-sequence-id-140106

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  16. KH001: pMVP (L3-L2) V5 epitope tag + WPRE

    Plasmid
    #121762
    Purpose
    pMVP L3-L2 entry plasmid, contains V5 epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    V5 epitope tag + WPRE
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121762-plasmid-map-sequence-id-242653

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  17. p3x-Flag Gli3 37R3-2

    Plasmid
    #120305
    Purpose
    Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37C
    Depositor
    David Liu
    Article
    Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi
    Insert
    Intein 37R3-2, Gli3
    Use
    Synthetic Biology
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Contains mutations as reported in 2011 Chemistry …
    Promoter
    CMV
    Available Since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    120305-plasmid-map-sequence-id-234346

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Showing: 5361 - 5380 of 7681 results