Skip to main content
Addgene
Showing: 1041 - 1080 of 4777 results
  1. pTRE3G-human cGAS:164-522aa-HA

    Plasmid
    #186859
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (164-522 a.a.) with C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pTRE3G-human cGAS:166-522aa-HA

    Plasmid
    #186860
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (166-522 a.a.) with C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pTRE3G-crab-eating macaque cGASdeltaN-HA

    Plasmid
    #186870
    Purpose
    Doxycycline-dependent expression of crab-eating macaque cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Crab-eating macaque cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (CGAS Macaca fascicularis, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pTRE3G-human cGASdeltaN-GFP

    Plasmid
    #186845
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with C-terminal GFP fusion (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    GFP
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pTRE3G-FLAG-human cGASdeltaN-HA

    Plasmid
    #186848
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    FLAG and HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pTRE3G-human cGASdeltaN C396/397A-HA

    Plasmid
    #186850
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with DNA binding mutantation C396/397A) and C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation, C396A, C397A
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pTRE3G-human cGAS:154-522aa-HA

    Plasmid
    #186855
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (154-522 a.a.) with C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

    Plasmid
    #129421
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein
    Depositor
    Insert
    TagBFP2-3Flag
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    3X Flag epitope tag
    Expression
    Mutation
    Promoter
    none (3X polyA to mitigate episomal expression)
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. pLenti-CMV-FLAG-orangutan cGASdeltaN-OMM

    Plasmid
    #186889
    Purpose
    Expression of orangutan cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with N-terminal FLAG tag and C-terminal MAVS transmembrane domain fusion (CGAS Pongo abelii, Synthetic)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    N-terminal truncation, MAVS transmembrane domain …
    Promoter
    CMV
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. MADR pDonor smFP-HA (dark) WPRE

    Plasmid
    #131007
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags
    Depositor
    Insert
    smFP-HA WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    HA - 10 total HA epitope tages
    Expression
    Mammalian
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. MADR pDonor smFP-V5 (dark) WPRE

    Plasmid
    #131006
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags
    Depositor
    Insert
    smFP-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    V5 - 10 total of V5 epitope tag
    Expression
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. MADR pDonor smFP-Myc (dark) WPRE

    Plasmid
    #130987
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags
    Depositor
    Insert
    smFP-Myc WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    Myc - 10 total Myc tags
    Expression
    Mutation
    "dark" variant with GGG fluorophore ver…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. MADR pDonor smFP-FLAG (dark) WPRE

    Plasmid
    #131005
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags
    Depositor
    Insert
    smFP-FLAG WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    FLAG - 10 total FLAG tags
    Expression
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131461
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53
    Depositor
    Insert
    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131462
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53
    Depositor
    Insert
    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A K27M, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131463
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53
    Depositor
    Insert
    H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A G34R, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 14, 2020
    Availability
    Academic Institutions and Nonprofits only
  17. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

    Plasmid
    #129420
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion
    Depositor
    Insert
    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    TagBFP2-V5 tag
    Expression
    Mutation
    G12V
    Promoter
    none (3x polyA to mitigate episomal expression)
    Available since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  18. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid
    #131730
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain
    Depositor
    Insert
    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    TagBFP2-V5 tag
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. pPI54

    Plasmid
    #113197
    Purpose
    extrachromosomal expression vector, N-terminal mTurquoise2 tagging
    Depositor
    Type
    Empty backbone
    Use
    Dictyostelium discoideum expression vector, extra…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  20. pPI142

    Plasmid
    #113198
    Purpose
    extrachromosomal expression vector, C-terminal mTurquoise2 tagging
    Depositor
    Type
    Empty backbone
    Use
    Dictyostelium discoideum expression vector, extra…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  21. pCFJ352

    Plasmid
    #30539
    Depositor
    Insert
    ttTi4348 region
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 29, 2011
    Availability
    Academic Institutions and Nonprofits only
  22. pCFJ210

    Plasmid
    #30538
    Depositor
    Insert
    ttTi4348 region
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 29, 2011
    Availability
    Academic Institutions and Nonprofits only
  23. pET28a(+)/FbGH17A

    Plasmid
    #100911
    Purpose
    Expression of the Beta-1,3-Endo-Laminarinase FbGH17A in E.coli
    Insert
    FbGH17A
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  24. pET28a(+)/FbGH17B

    Plasmid
    #100912
    Purpose
    Expression of the Beta-1,3-Exo-Laminarinase FbGH17B in E.coli
    Insert
    FbGH17Bcat
    Use
    Tags
    His-Tag
    Expression
    Bacterial
    Mutation
    not full length
    Promoter
    Available since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
  25. pDONR221-RBM11

    Plasmid
    #192748
    Purpose
    Gateway entry vector encoding human RBM11
    Depositor
    Insert
    RBM11 (RBM11 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  26. PB{3xP3-dsRed2; β2Tubulin-mNeonGreen}

    Plasmid
    #236085
    Purpose
    PiggyBac plasmid for the insertion of a sperm-expressed green fluorescent protein in Aedes aegypti
    Depositor
    Insert
    3xP3-dsRed2; β2Tubulin-mNeonGreen
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  27. pDONR221-mis18a

    Plasmid
    #192747
    Purpose
    Gateway entry vector encoding zebrafish mis18a
    Depositor
    Insert
    mis18a (si:dkey-189i22.1 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  28. pDONR221-chodl

    Plasmid
    #192738
    Purpose
    Gateway entry vector encoding zebrafish chodl
    Depositor
    Insert
    chodl (zgc:110088 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    S192I
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  29. pDONR221-ncam2

    Plasmid
    #192739
    Purpose
    Gateway entry vector encoding zebrafish ncam2
    Depositor
    Insert
    ncam2 (ncam2 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  30. pDONR221-sh3bgr

    Plasmid
    #192727
    Purpose
    Gateway entry vector encoding zebrafish sh3bgr
    Depositor
    Insert
    sh3bgr (zgc:100896 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  31. pDONR221-olig1

    Plasmid
    #192744
    Purpose
    Gateway entry vector encoding zebrafish olig1
    Depositor
    Insert
    olig1 (olig1 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    F106L
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  32. pDONR221-appb

    Plasmid
    #192746
    Purpose
    Gateway entry vector encoding zebrafish appb
    Depositor
    Insert
    appb (appb Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    D233E (rs508877069)
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  33. pDONR221-dyrk1ab

    Plasmid
    #192726
    Purpose
    Gateway entry vector encoding zebrafish dyrk1ab
    Depositor
    Insert
    dyrk1ab (dyrk1ab Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    G685S
    Promoter
    None
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  34. pDONR221-get1

    Plasmid
    #192722
    Purpose
    Gateway entry vector encoding zebrafish get1
    Depositor
    Insert
    get1 (wrb Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  35. pDONR221-hmgn6

    Plasmid
    #192715
    Purpose
    Gateway entry vector encoding zebrafish hmgn6
    Depositor
    Insert
    hmgn6 (hmgn6 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  36. Nup133_mEGFP(-8a)

    Plasmid
    #163417
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)
    Depositor
    Insert
    Nup133 (NUP133 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  37. pDONR221-olig2

    Plasmid
    #192743
    Purpose
    Gateway entry vector encoding zebrafish olig2
    Depositor
    Insert
    olig2 (olig2 Zebrafish)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    S61A (rs501471300); G235S (rs505716173)
    Promoter
    None
    Available since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 1041 - 1080 of 4777 results