We narrowed to 9,252 results for: SUB

1. ylqF-pET21a

   Plasmid

   #30133

   Depositor

   SeWon Suh

   Article

   Kim do et al Proteins. 2008 Sep . 72(4):1363-70.

   Insert

   ylqF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pMG36E

   Plasmid

   #133853

   Purpose

   Expression vector

   Depositor

   Jan Kok, Gerard Venema

   Article

   van de Guchte et al Appl Environ Microbiol. 1989 Jan;55(1):2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P32

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-sfGFP

   Plasmid

   #125747

   Purpose

   sfGFP without iCreERT2 fusion

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTU2-AmyE-Pveg-eGFP

   Plasmid

   #112792

   Purpose

   Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation

   Depositor

   Chris French

   Article

   Wicke et al bioRxiv 185108

   Insert

   Pveg-eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLSSmKate2-N1

   Plasmid

   #31867

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   LSS-mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167D/T183S/M196V

   Promoter

   CMV

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 3xF-dyskerin

   Plasmid

   #126870

   Purpose

   Expresses 3xFlag dsykerin

   Depositor

   Kathleen Collins

   Article

   Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

   Insert

   Dyskerin
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGWT35S-mCherry

   Plasmid

   #182582

   Purpose

   Transient expression of mCherry in plant cell (Cytosol)

   Depositor

   Yutaka Kodama

   Article

   Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDH-mApple

   Plasmid

   #179383

   Purpose

   Lentiviral vector for expression of mApple

   Depositor

   Angus Johnston

   Article

   Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

   Insert

   mApple
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ActTS-GR

   Plasmid

   #79774

   Purpose

   FRET-based actinin tension sensors

   Depositor

   Tetsuya Kitaguchi

   Article

   Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

   Insert

   ActTS-GR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Su9-EGFP

    Plasmid

    #23214

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Subunit 9 of the F0-ATPase
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains AA 1-69 of subunit 9

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pB-Rex

    Plasmid

    #122134

    Purpose

    B. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 origin

    Depositor

    Srivatsan Raman

    Article

    Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

    Insert

    B. subtilis Rex
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    apFAB338

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSP955

    Plasmid

    #98784

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proB-lysozyme-ggs-ENLYFQS-ggs-T7RNAP//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GST-PRMT7

    Plasmid

    #34693

    Depositor

    Steven Clarke

    Article

    Miranda et al J Biol Chem. 2004 May 28;279(22):22902-7

    Insert

    PRMT7 (PRMT7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pSR1058

    Plasmid

    #208743

    Purpose

    Characterization plasmid for PHbs (sfgfp expressed from PHbs on the S. aureus pC194 replicon)

    Depositor

    Lauren Andrews

    Article

    Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

    Insert

    PHbs
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PHbs

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLRD18

    Plasmid

    #208270

    Purpose

    Production of ent-isokaurene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    ptmT2
    osKSL6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDR-GW AT1.03

    Plasmid

    #28003

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    AT1.03
    

    Use

    Tags

    mVenus and mseCFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCG004

    Plasmid

    #87377

    Purpose

    ORF part assembly entry vector derived from pHT01.

    Depositor

    Tom Ellis

    Article

    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

    Insert

    GFP dropout part
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPICZalphaB-SapL3

    Plasmid

    #78171

    Purpose

    Expression of Saporin L3 in pichia

    Depositor

    Vern Schramm

    Article

    Yuan et al Biochemistry. 2015 Jul 28;54(29):4565-74

    Insert

    Saporin L3
    

    Use

    Tags

    alpha factor

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HCMV UL97

    Plasmid

    #26687

    Depositor

    Robert Kalejta

    Article

    Kuny et al PLoS Pathog. 2010 . 6(9):.

    Insert

    UL97 (UL97 Human Cytomegalovirus (HHV-5))
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pJ241-FLEX

    Plasmid

    #18925

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    FLEX switch
    

    Use

    Cre/Lox; Subcloning

    Tags

    Expression

    Mutation

    An internal Spe1 site is used for subcloning a ge…

    Promoter

    Available since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HHV7 U69

    Plasmid

    #26693

    Depositor

    Robert Kalejta

    Article

    Kuny et al PLoS Pathog. 2010 . 6(9):.

    Insert

    U69 (U69 Human Herpes Virus 7)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

22. HHV6 U69

    Plasmid

    #26689

    Depositor

    Robert Kalejta

    Article

    Kuny et al PLoS Pathog. 2010 . 6(9):.

    Insert

    U69 (U69 Human Herpes Virus 6)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pFastBac1 Flag Baf170

    Plasmid

    #1955

    Depositor

    Robert Kingston

    Article

    Phelan et al Mol Cell 1999 Feb;3(2):247-53.

    Insert

    BAF170 (SMARCC2 Human)
    

    Use

    Tags

    flag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pAV1825

    Plasmid

    #37838

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Pnhx-2DsRed::KDEL
    

    Use

    Tags

    KDEL

    Expression

    Worm

    Mutation

    Promoter

    nhx-2

    Available since

    Dec. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pAG306-pTet07-nLuc

    Plasmid

    #199755

    Purpose

    Control construct of elongation reporter to quantify elongation of YFP

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    Nanoluc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-BRIC

    Plasmid

    #172338

    Purpose

    Bioluminescent Red Indicator for Calcium

    Depositor

    Huiwang Ai

    Article

    Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

    Insert

    BRIC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pME-mCherry-24xMBS

    Plasmid

    #86242

    Purpose

    Middle entry vector for generating MBS tagged RNAs with a fluorescent protein reporter

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    mCherry-24xMBS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD-BRIC

    Plasmid

    #172343

    Purpose

    Bioluminescent Red Indicator for Calcium (bacterial expression)

    Depositor

    Huiwang Ai

    Article

    Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

    Insert

    BRIC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJW1664

    Plasmid

    #112038

    Purpose

    CEN/ARS plasmid expression of mitochondrial Su9-TagBFP marked with HIS

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    Su9
    

    Use

    Tags

    TagBFP

    Expression

    Yeast

    Mutation

    amino acids 1-76

    Promoter

    Available since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJW1663

    Plasmid

    #112037

    Purpose

    Gal-Estradiol transcription activation expression cassette marked with KanR

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    Gal DNA binding-estradiol binding-MSN2 activator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJW1666

    Plasmid

    #112040

    Purpose

    CEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    None
    

    Use

    Tags

    V5-TEV-EGFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. MSP782

    Plasmid

    #98790

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proB-lysozyme-ggs-HPLVGHM-ggs-T7RNAP//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. dBrainbow

    Plasmid

    #29327

    Depositor

    Julie Simpson

    Article

    Hampel et al Nat Methods. 2011 Feb 6. ():.

    Insert

    UAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLV.CMV.GFP.miR124.sponge

    Plasmid

    #115975

    Purpose

    Kockdown of miR-124

    Depositor

    Johan Jakobsson

    Article

    Akerblom et al J Neurosci. 2012 Jun 27;32(26):8879-89.

    Insert

    GFP-miR124sponge
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AND-gate-V2.0

    Plasmid

    #44449

    Depositor

    Drew Endy

    Article

    Bonnet et al Science. 2013 May 3;340(6132):599-603. d

    Insert

    AND-gate
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MSP577

    Plasmid

    #98801

    Purpose

    Expression Vector

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    pET28 MBP-HNLYFQS-TEV I138T, N171D, N176T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I138T, N171D, N176T

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMN cFKT

    Plasmid

    #18785

    Depositor

    Han Htun

    Article

    Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

    Insert

    TRAP (mtrB Bacillus subtilis)
    

    Use

    Tags

    6xHis and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSR1048

    Plasmid

    #208738

    Purpose

    Characterization plasmid for PRelA (sfgfp expressed from PRelA on the S. aureus pC194 replicon)

    Depositor

    Lauren Andrews

    Article

    Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

    Insert

    PRelA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PRelA

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MSP565

    Plasmid

    #98785

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proB-lysozyme-ggs-HNLYFQS-ggs-T7RNAP//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MSP780

    Plasmid

    #98788

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proB-lysozyme-ggs-HNLYGHS-ggs-T7RNAP//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. MSP794

    Plasmid

    #98789

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proB-lysozyme-ggs-HNLVGHS-ggs-T7RNAP//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pJW1660

    Plasmid

    #112034

    Purpose

    C-terminal tag with AID*(AtIAA17_71-114)-HA marked with LEU

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    IAA17
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    only amino acids 71-114

    Promoter

    None

    Available since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pXM03

    Plasmid

    #121150

    Purpose

    This plamid was used for biosynthesis of Prenylated 4‑Hydroxybenzoate in E.coli

    Depositor

    Minjuan Xu

    Article

    He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

    Insert

    XimB, IDS2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. p2Bac.V5.p62

    Plasmid

    #39218

    Depositor

    Aziz Sancar

    Insert

    p62 (GTF2H1 Human)
    

    Use

    Tags

    V5

    Expression

    Insect

    Mutation

    Promoter

    Pp10

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

45. PMT-mApple

    Plasmid

    #203771

    Purpose

    Brightness calibration

    Depositor

    Thorsten Wohland

    Article

    Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

    Insert

    PMT-mApple
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pRBUBR1

    Plasmid

    #20198

    Depositor

    Alexander Varshavsky

    Article

    Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

    Insert

    UBR1 (UBR1 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

47. MSP770

    Plasmid

    #98787

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proB-lysozyme-ggs-HNLYFHS-ggs-T7RNAP//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. MSP848

    Plasmid

    #98795

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proA-lysozyme-IL-23(38-66)-T7RNAP(Q649S)//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. MSP722

    Plasmid

    #98786

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proB-lysozyme-ggs-ENLYGQS-ggs-T7RNAP//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pAV1951

    Plasmid

    #37832

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Pnhx-2mCherry
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    nhx-2

    Available since

    Nov. 27, 2012

    Availability

    Academic Institutions and Nonprofits only