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UAS-u(XS)Cas9

Plasmid

#127382

Purpose

Expresses Cas9 at very high level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF-Cas9

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4 human alpha5 full

Plasmid

#221396

Purpose

Mammalian expression of human integrin alpha5 full length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 full length (ITGA5 Human)

Use

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and St…

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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PD2529 human alpha5 full

Plasmid

#221395

Purpose

Mammalian expression of human integrin alpha5 full length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 full length (ITGA5 Human)

Use

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA)

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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UAS-FRT-GFP-FRT-u(M)Cas9

Plasmid

#127388

Purpose

Cas9 inducible by removal of a GFP flip-out cassette

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

FRT-GFP-FRT-uORF(M)Cas9

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-3xHA-V5-RpL10Ab

Plasmid

#125223

Purpose

3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Use

Tags

3x HA, V5

Expression

Insect

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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mTFP1-Alpha-V-Integrin-25

Plasmid

#55465

Purpose

Localization: Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Alpha-V Integrin (ITGAV Human)

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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mEos2-Alpha-V-Integrin-25

Plasmid

#57345

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Catherine Galbraith

Article

Jaqaman et al Mol Biol Cell. 2016 May 15;27(10):1561-9

Insert

Alpha-V Integrin (ITGAV Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
ITGAV-bio-His

Plasmid

#51919

Purpose

Expresses full-length Integrin alpha-V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ITGAV (ITGAV Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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ITGA5-bio-His

Plasmid

#51909

Purpose

Expresses full-length Integrin alpha-5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ITGA5 (ITGA5 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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mouse a5 full length

Plasmid

#217810

Purpose

Full length mouse integrin a5 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGA5 (Itga5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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miniSOG-Alpha-V-Integrin-25

Plasmid

#57763

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Alpha-V Integrin (ITGAV Human)

Use

Tags

miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PD2529 human alpha5 ecto

Plasmid

#221394

Purpose

Mammalian expression of human integrin alpha5 ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 ectodomain (ITGA5 Human)

Use

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HR…

Expression

Mammalian

Mutation

codon optimized for human mature residues _5 F1 t…

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCAD29_hIRG1_4-461_pvp008

Plasmid

#124843

Purpose

Expresses human CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Use

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 462-end.

Promoter

T7

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAD39_mIRG1_4-462_pvp008

Plasmid

#124873

Purpose

Expresses mouse CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (Acod1 Mouse)

Use

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 463-end.

Promoter

T7

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAD104_Asn152Ser_pCMV6Entry-hIRG1

Plasmid

#124877

Purpose

Expresses hCAD Asp152Ser mutant in mammalian cells

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Use

Tags

myc and FLAG tags

Expression

Mammalian

Mutation

mutated Asn152 to Ser

Promoter

CMV

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAD64_pCMV6_Entry_hIrg1

Plasmid

#124879

Purpose

Expresses hCAD His103Ala mutant in mammalian cells

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Use

Tags

myc and FLAG tags

Expression

Mammalian

Mutation

mutated His103 to Ala

Promoter

CMV

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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human a5 full length

Plasmid

#217818

Purpose

Full length human integrin a5 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGA5 (ITGA5 Human)

Use

Tags

P2A-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCAD54_CAD29_H104A_pvp008

Plasmid

#124876

Purpose

Expresses hCAD His103Ala mutant in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Use

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

mutated His103 to Ala, deleted amino acids 1-3 an…

Promoter

T7

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAD108_Arg273His_pCMV6Entry-hIRG1

Plasmid

#124878

Purpose

Expresses hCAD Arg273His mutant in mammalian cells

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Use

Tags

myc and FLAG tags

Expression

Mammalian

Mutation

mutated Arg273 to His

Promoter

CMV

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAD96_hIRG1_4-461_pvp008

Plasmid

#124874

Purpose

Expresses hCAD Asn152Ser mutant in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Use

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

mutated Asn152 to Ser, deleted amino acids 1-3 an…

Promoter

T7

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge