We narrowed to 12,075 results for: ache

1. pHAGE2-EF1aL-Cre-IRES-NeoR-W

   Plasmid

   #126700

   Purpose

   lentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassette

   Depositor

   Darrell Kotton

   Article

   Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

   Insert

   Cre-IRES
   

   Use

   Cre/Lox and Lentiviral

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti CMV Puro DEST (w118-1) PHLDB1

   Plasmid

   #107503

   Purpose

   Expresses PHLDB1, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   PHLDB1 (PHLDB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Nucleotide changes 1236 T>C, 1365 C>T, 1371…

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLgw ELK4-V5-EcoDam

   Plasmid

   #98607

   Purpose

   Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK4 (Elk4 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   V5 and Dam (DNA adenine methyltransferase)

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPHR-mCherry-hNCL

   Plasmid

   #181886

   Purpose

   Soluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-hNCL (NCL Human, Mustard Weed)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti CMV Puro DEST (w118-1) MXRA7

   Plasmid

   #107500

   Purpose

   Expresses MXRA7, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   MXRA7 (codon optimized) (MXRA7 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized sequence

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a-TALEN_HD (SPC right)

   Plasmid

   #126695

   Purpose

   right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

   Depositor

   Darrell Kotton

   Article

   Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

   Insert

   human SPC right
   

   Use

   TALEN

   Tags

   NLS, truncated N term and TAL, FokI

   Expression

   Mammalian

   Mutation

   R451P in SPC

   Promoter

   EF1

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a-TALEN_NN (SPC left)

   Plasmid

   #126694

   Purpose

   left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

   Depositor

   Darrell Kotton

   Article

   Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

   Insert

   human SPC left
   

   Use

   TALEN

   Tags

   0.5 repeat NN, TAL, FokI and NLS, truncated N ter…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV Puro DEST (w118-1) VOPP1

   Plasmid

   #107507

   Purpose

   expressing VOPP1, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   VOPP1 (VOPP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

   Plasmid

   #169168

   Purpose

   Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Use

   Tags

   6X Histidine Tag, TEV cleavage site, mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac promoter

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV Puro DEST (w118-1) RALB

    Plasmid

    #107506

    Purpose

    Expressing RALB, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    RALB (RALB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSumo24P7vB4

    Plasmid

    #113073

    Purpose

    Plasmid for highly efficient expression of engineered IL24 with binding affinity to cognate receptors

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

    Insert

    Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 Human, SUMO part (Brachypodium distachyon))
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSumo24P7

    Plasmid

    #113072

    Purpose

    Plasmid for highly efficient expression of engineered IL24 with mutated binding sites

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al FEBS J. 2019 Oct;286(19):3858-3873. doi:

    Insert

    Human IL-24 engineered by computational design and fused with N-terminal SUMO tag (IL24 Human, SUMO part (Brachypodium distachyon))
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Q60R, K62E, K68E, K77R, M80L, S88D, A89V, Q93R, Q…

    Promoter

    T7

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGFP-nE-mHP1alpha

    Plasmid

    #181895

    Purpose

    Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    nE-mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGFP-nE-mHP1alpha-delCTE

    Plasmid

    #181896

    Purpose

    Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    nE-mHP1alpha-delCTE (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGFP-FUSN-mHP1alpha

    Plasmid

    #181897

    Purpose

    Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    FUSN-mHP1alpha (Cbx5 Human, Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGFP-PGL-1-mHP1alpha

    Plasmid

    #181899

    Purpose

    Fluorescently tagged HP1alpha fused to PGL-1

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PGL-1-mHP1alpha (Cbx5 Nematode, Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRGG-GFP-RGG-mHP1alpha

    Plasmid

    #181898

    Purpose

    Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    RGG-GFP-RGG (Cbx5 Nematode, Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti CMV Puro DEST (w118-1) TSPAN9

    Plasmid

    #107502

    Purpose

    Expresses TSPAN9, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    TSPAN9 (TSPAN9 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLgw EcoDam-V5-ELK4

    Plasmid

    #98606

    Purpose

    Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK4 (Elk4 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid

    #232116

    Purpose

    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    BCKDK (BCKDK Human)
    

    Use

    Tags

    6 x His, Biotin acceptor peptide (BAP), Maltose-B…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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