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pBW1997_pExpr-hU6-CRISPR-DECODER

Plasmid

#87549

Purpose

Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

gRNAs targeting NGN2, MIAT, ACTC1, TTN

Use

CRISPR, Cre/Lox, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G13 mutant

Plasmid

#231915

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G13 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1158Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G14 mutant

Plasmid

#231916

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G14 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1173Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G15 mutant

Plasmid

#231917

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G15 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1194Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G145 mutant

Plasmid

#231918

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G145 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1173Q, N1194Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G1 mutant

Plasmid

#231913

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G1 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G12 mutant

Plasmid

#231914

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G12 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1134Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSCL758

Plasmid

#219504

Purpose

Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences

Use

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-HO-G

Plasmid

#164163

Purpose

Expresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mEGFP A236K

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-ePsuACR-mRuby2-ST

Plasmid

#109133

Purpose

Anion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ePsuACR-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-eiC++-mRuby2-ST

Plasmid

#109132

Purpose

Artificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eiC++-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pWN10042

Plasmid

#89052

Purpose

GF-ori-FP plasmid for GFxFP assay

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

EGFP part 1
EGFP part 2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG and none

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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Tg 2 kb Violet opsin GFP

Plasmid

#72921

Purpose

violet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving GFP

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

violet opsin promoter

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Tg 2 kb Violet opsin DsRed

Plasmid

#72920

Purpose

violet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving DsRed

Depositor

Joseph Corbo

Article

Enright et al J Comp Neurol. 2015 Mar 1;523(4):649-68.

Insert

violet opsin promoter

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pWN_U6-B2M-miniGag-Cas9

Plasmid

#228958

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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T/brachyury(2A-Dre)

Plasmid

#140761

Purpose

Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

Dre

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Endogenous T/brachyury

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pWN_U6-TRAC-miniGag-Cas9

Plasmid

#228959

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEX-PK-hLC3

Plasmid

#61458

Purpose

Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy

Depositor

Isei Tanida

Article

Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

Insert

PK-hLC3 (MAP1LC3B Fission Yeast, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG dCas9-KRAB-2A-EGFP

Plasmid

#92396

Purpose

CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9_KRAB_2A_EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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