We narrowed to 12,018 results for: cel.2;

1. PAmCherry-N1-FRB-Tubbyc

   Plasmid

   #202678

   Purpose

   Photoactivatable and rapamycin recruitable lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   Mus musculus Tub (243-505):MTOR(2021-2113):PAmCherry1 (Tub, Mtor Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EYFP-NLS-actin-K68/284R

   Plasmid

   #60616

   Purpose

   Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

   Depositor

   Primal de Lanerolle

   Article

   Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

   Insert

   Beta-actin (ACTB Human)
   

   Use

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   K68R, 284R

   Promoter

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pRSETa-mEos3.2-A69T

   Plasmid

   #98571

   Purpose

   photoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mEos3.2-A69T
   

   Use

   Tags

   7xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1 p18 mito

   Plasmid

   #26638

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   p18 mito (LAMTOR1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

5. PAmCherry1-N1-Lyn11

   Plasmid

   #202683

   Purpose

   Photoactivatable lipid biosensor

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   LYN(1-11):PAmCherry1 (LYN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a-VHH-tev-APEX2

   Plasmid

   #117751

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP, tev cleavage site and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

   Insert

   anti-GFP nanobody fused to a T7, tev site, APEX2, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-Lmo2

   Plasmid

   #60666

   Purpose

   PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

   Depositor

   James Thomson

   Article

   Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

   Insert

   Lmo2 (Lmo2 Mouse)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKM60

   Plasmid

   #154159

   Purpose

   Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   4 wt NF-κB sites were replaced with Rel1A-specifi…

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. XE123 XDsh delta PDZ-GFP- CS2+

   Plasmid

   #16786

   Depositor

   Randall Moon

   Article

   Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

   Insert

   Xdsh-delta-PDZ (dvl2.L Frog)
   

   Use

   Xenopus expression

   Tags

   gfp

   Expression

   Mutation

   contains Dsh amino acids 1-267 and 381-736 (delet…

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pRSETa-mEos4b-V69T

    Plasmid

    #98572

    Purpose

    photoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    mEos4b-V69T
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pminiT-Halo-MED1 donor

    Plasmid

    #197868

    Purpose

    Donor plasmid used for building Halo-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Halotag

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSETa-mKikGR-V69T

    Plasmid

    #98574

    Purpose

    photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    mKikGR-V69T
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-sgRNA_Dicer_3

    Plasmid

    #68809

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSUPER.retro BRIT1

    Plasmid

    #16206

    Depositor

    Shiaw-Yih Lin

    Article

    Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

    Insert

    shBRIT1 (MCPH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

15. XE157 GFP-XDpr-delta PDZB-CS2+

    Plasmid

    #16765

    Depositor

    Randall Moon

    Article

    Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

    Insert

    XDpr-delta PDZB (dact1-b Frog)
    

    Use

    Xenopus expression

    Tags

    gfp

    Expression

    Mutation

    XDpr is truncated to delete the last four amino a…

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3 HRPT2 L64P

    Plasmid

    #11052

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 L64P (CDC73 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L64P

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-EngPD

    Plasmid

    #64039

    Purpose

    Mammalian expression of paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)

    Depositor

    Daniel Christophe

    Article

    Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2

    Insert

    Pax8 paired domain (Pax8 Mouse)
    

    Use

    Tags

    Eng (N-terminal repressor domain of the drosophi…

    Expression

    Mammalian

    Mutation

    paired domain, aa 1-147

    Promoter

    CMV

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

18. XE125 XDsh delta COOH-GFP-CS2+

    Plasmid

    #16784

    Depositor

    Randall Moon

    Article

    Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

    Insert

    Xdsh-delta-COOH (dvl2.L Frog)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    contains Dsh amino acids 1-588 (deleting 599-736)

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

19. KHBD00327

    Plasmid

    #34450

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG18783 (Kr-h1 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pminiT-mEmerald-MED1 donor

    Plasmid

    #197867

    Purpose

    Donor plasmid used for building mEmerald-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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