We narrowed to 154,128 results for: ins;

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pBabe-PI(WT)-Src(KD)-mCherry

Plasmid

#87358

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Src (Src Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

D388R

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX SHIP2-SH2

Plasmid

#46493

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHIP2

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 8-131

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-2

Plasmid

#11371

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
3xFKBP-myc

Plasmid

#20213

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFKBP (FKBP1A Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pGEX Syk(NC)-SH2

Plasmid

#46521

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(NC) (SYK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 7-263

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGen2.1-redCALWY-1

Plasmid

#50739

Purpose

Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

Depositor

Maarten Merkx

Article

Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

Insert

gene for redCALWY-1 Zn2+ sensor

Expression

Mammalian

Available Since

Jan. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pJRoC30_VP5H

Plasmid

#188061

Purpose

PROSS-designed versatile peroxidase 5H

Depositor

Sarel Fleishman

Article

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Pleurotus ostreatus

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

43 PROSS mutations, described in publication

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK4-S

Plasmid

#172425

Purpose

Expression of full-length, human GIRK4 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK4 (KCNJ5 Human)

Tags

Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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MSP2279

Plasmid

#70706

Purpose

Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

D10A and H557A in SaCas9

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pEAK-bKal7

Plasmid

#145800

Purpose

expresses Kalirin-7 with the b-front in mammalian cells

Depositor

Betty Eipper, Richard Mains

Article

Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

Insert

bKalirin-7 (Kalrn Rat)

Expression

Mammalian

Promoter

EIF2a

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pFUGW-RapACR-EYFP

Plasmid

#111075

Purpose

Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RapACR

Use

Lentiviral

Tags

EYFP

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-MPHOSPH8 Chromo

Plasmid

#59694

Purpose

Contains histone modification interacting domain (MPHOSPH8 Chromo)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

MPHOSPH8 Chromo (MPHOSPH8 Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pFUGW-RapACR_T111C-EYFP

Plasmid

#119195

Purpose

Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin RapACR fast mutant T111C

Use

Lentiviral

Tags

EYFP

Mutation

changed threonine 111 to cysteine

Promoter

Ubiquitin

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(C)-SH2

Plasmid

#46469

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(C)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 656-766

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET28 sf:mid.11

Plasmid

#191921

Purpose

Encodes a GFP design, sf:mid.11

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:mid.11

Tags

His Tag

Expression

Bacterial

Mutation

Q69M Y145F T167I L220V V224I

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:mid.7

Plasmid

#191933

Purpose

Encodes a GFP design, sf:mid.7

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:mid.7

Tags

His Tag

Expression

Bacterial

Mutation

S72T Y145F V224I

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 acid.6

Plasmid

#191912

Purpose

Encodes a GFP design, acid.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.6

Tags

His Tag

Expression

Bacterial

Mutation

Q69L S72A Y145M T167V H181L

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:acid.3

Plasmid

#191926

Purpose

Encodes a GFP design, sf:acid.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:acid.3

Tags

His Tag

Expression

Bacterial

Mutation

T65S Q69L S72A T108V Y145M V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.7

Plasmid

#191900

Purpose

Encodes a GFP design, mid.7

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.7

Tags

His Tag

Expression

Bacterial

Mutation

S72T Y145F V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLTR PKC beta II

Plasmid

#8417

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Rat Protein Kinase C beta II (Prkcb Rat)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only