We narrowed to 9,265 results for: yeast expression

1. pJT43_GalL_BE-P(AP)3

   Plasmid

   #145036

   Purpose

   Expresses BE-P(AP)3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   BE-(AP)3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS405 PEX14-VAC8-RFP

   Plasmid

   #166844

   Purpose

   Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8, PEX14 Budding Yeast)
   

   Use

   Tags

   PEX14 transmembrane domain and RFP

   Expression

   Yeast

   Mutation

   PEX14 transmembrane domain

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB1975 (pUC19-PTEF1-PPGK1)

   Plasmid

   #73296

   Purpose

   Example plasmid containing a bidirectional promoter

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Insert

   pTEF1-pPGK1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX4T2-Snf1-cat

   Plasmid

   #52683

   Purpose

   expression of the Snf1 kinase domain in bacteria

   Depositor

   Benjamin Turk

   Article

   Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

   Insert

   SNF1 (SNF1 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes kinase domain only, aa 41-315

   Promoter

   tac

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pRS406 ATG1-ATG17-3'UTR

   Plasmid

   #166848

   Purpose

   Expresses Atg1 with ATG17 3'UTR in yeasts.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

   Insert

   ATG1 (ATG1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ATG1 Promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS316-RGR-GFP-mm

   Plasmid

   #51059

   Purpose

   Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   RGR-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJKW1847

   Plasmid

   #174004

   Purpose

   Expresses PpCYP94D108 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

   Depositor

   Jing-Ke Weng

   Article

   Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

   Inserts

   PpCYP94D108
   ATR1 (ATR1 Mustard Weed)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW3.42

   Plasmid

   #62866

   Purpose

   Yeast two-hybrid bait vector expressing LexA-PPTR-1 fusion

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   PPTR-1/pLexA-N bait
   

   Use

   Two-hybrid bait vector

   Tags

   LexA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

   Plasmid

   #216404

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Alanine 51, 56, Glutamine 70, 443 and Asp…

   Promoter

   Available Since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57-fsr1

    Plasmid

    #179866

    Purpose

    Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiae

    Depositor

    Jens Laurids Sørensen

    Article

    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

    Insert

    Fusarubin polyketide synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Codon optimized for expression in Saccharomyces c…

    Promoter

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTF1 deltaC12

    Plasmid

    #205068

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 12 amino acids

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTF1 deltaC16

    Plasmid

    #205069

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 16 amino acids

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTF1 deltaC18

    Plasmid

    #205070

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 18 amino acids

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTF1 deltaC20

    Plasmid

    #205071

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 20 amino acids

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. MTF1 deltaC9

    Plasmid

    #205067

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 9 amino acids

    Promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBT9-tepsin

    Plasmid

    #196647

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-tepsin fusion protein

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    tepsin (TEPSIN Human)
    

    Use

    Yeast two-hybrid vector

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    silent substitution in codon 199 to eliminate int…

    Promoter

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Linked mRuby3 VLP

    Plasmid

    #182430

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-GFP-mRuby3
    deltaVP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Linked Free NES (M)

    Plasmid

    #182436

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Insert

    FPPS(M)-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJCble

    Plasmid

    #185861

    Purpose

    The CRISPR/Cas9-and-gRNA-expressing plasmid with a phleomycin-resistent selectable marker

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; ble
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. p406 – TDH3-MTS-GFP11-Ura

    Plasmid

    #177733

    Purpose

    Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA marker

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    MTS-GFP11
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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