159,284 results

1. pMH-SFB

   Plasmid

   #99391

   Purpose

   Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Use

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-Int

   Plasmid

   #50000

   Purpose

   Expresses the integrase on a suicide plasmid

   Depositor

   Brian Robertson, Peter Sander

   Article

   Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

   Insert

   Integrase from pMV361
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   own

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pRDA_174

   Plasmid

   #136476

   Purpose

   Lentiviral expression of EnAsCas12a

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   EnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Vimentin - APEX2 in pECFP

   Plasmid

   #66170

   Purpose

   APEX2 fused to the C-terminus human vimentin

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   human Vimentin - APEX2 (VIM Synthetic, Human)
   

   Use

   Tags

   Flag epitope tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   APEX2 contains 4 mutations relative to wt soybean…

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD_Kan-LacI

   Plasmid

   #79826

   Purpose

   Arabinose induced expression of Lac repressor

   Depositor

   Katja Arndt

   Article

   Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

   Insert

   LacI
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   araBp

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-SFFV-KRAB-dCas9-P2A-mCherry

   Plasmid

   #60954

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Insert

   KRAB-dCas9-P2A-mCherry fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, KRAB domain, and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE60-EFG-His

   Plasmid

   #124126

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Elongation factor G (fusA Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-CMV-Galpha-s ONE-GO

   Plasmid

   #189731

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-s ONE-GO
   GNAS (GNAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC189

   Plasmid

   #32114

   Depositor

   Eric Rubin

   Article

   Chiang et al Gene. 2002 Aug 21;296(1-2):179-85.

   Type

   Empty backbone

   Use

   Transposon delivery vector

   Tags

   3x FLAG

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-puro-IMS-RP

    Plasmid

    #24535

    Depositor

    Peter Sorger

    Article

    Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

    Insert

    Diablo homolog (DIABLO Human)
    

    Use

    Retroviral

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Deleted residues 56-239

    Promoter

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. ABE8.20-NG

    Plasmid

    #226857

    Purpose

    Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. nuc-mCherry-pTRIP

    Plasmid

    #163520

    Purpose

    Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

    Depositor

    Thomas Weber

    Insert

    nucleus targeted mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV Puro-CMV:GFP

    Plasmid

    #68485

    Purpose

    Retrovirus expressing GFP driven by CMV promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    Puromycin Resistance
    GFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and LTR

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-ATAD2

    Plasmid

    #65370

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    ATAD2 (ATAD2 Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF0978

    Plasmid

    #141775

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF7 (KLF7 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Green Glifon600

    Plasmid

    #126207

    Purpose

    green fluorescent glucose sensor for mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Mita et al Anal Chem. 2019 Apr 2;91(7):4821-4830. d

    Insert

    Green Glifon600
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCherry.90 alpha

    Plasmid

    #108222

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 alpha

    Depositor

    Didier Picard

    Article

    Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

    Insert

    mCherry-Hsp90a (HSP90AA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET303-colE1T

    Plasmid

    #155180

    Purpose

    Expresses colicin E1 residues 1-190

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    colicin E1 residues 1-190
    

    Use

    Tags

    C-terminal 6x Histidine tag

    Expression

    Bacterial

    Mutation

    truncation of colicin E1 including 1-190

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pN1-GCaMP6m-XC

    Plasmid

    #111543

    Purpose

    Improved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-X

    Depositor

    Xiaodong Liu

    Article

    Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

    Insert

    GCaMP6m-XC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hPDGFRA-GFP

    Plasmid

    #22919

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 . 3():19.

    Insert

    human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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