161,570 results

1. pcDNA3.1-3xFLAG-V5-ccdB

   Plasmid

   #87064

   Purpose

   Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

   Depositor

   Mikko Taipale, Susan Lindquist

   Article

   Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

   Type

   Empty backbone

   Use

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. LAMP1-Venus

   Plasmid

   #234750

   Purpose

   Mammalian expression of lysosome marker LAMP1-Venus

   Depositor

   Nevin Lambert

   Article

   Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

   Insert

   LAMP1-Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Venus-2xFYVE

   Plasmid

   #234746

   Purpose

   Mammalian expression of endosome marker Venus-2xFYVE

   Depositor

   Nevin Lambert

   Article

   Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

   Insert

   Venus-2xFYVE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. ERM-APEX2

   Plasmid

   #79055

   Purpose

   Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 anchored to the cytosolic face of the ER membrane
   

   Use

   Lentiviral

   Tags

   Residues 1-27 of P450 oxidase 2C1 and V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV immearly promotor

   Available since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-SFFV-GFP1-10

   Plasmid

   #80409

   Purpose

   Expresses GFP1-10 in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP1-10
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. mpeg:lamp1b-mCherry-stop; cmlc2:GFP

   Plasmid

   #213739

   Purpose

   Expresses Lamp1b-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

   Depositor

   William Talbot

   Article

   Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

   Insert

   lamp1b-mCherry
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   mpeg1.1

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPD2

   Plasmid

   #68161

   Purpose

   Universal domesticator plasmid for level 0 parts

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP- SEC61B

   Plasmid

   #121159

   Purpose

   Mammalian expression of GFP tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. LeGO-V2

   Plasmid

   #27340

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   SFFV promoter, Venus fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEB1-SCFP3A

    Plasmid

    #103970

    Purpose

    Plasmid encoding SCFP3A

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    SCFP3A
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GIPR-DuET

    Plasmid

    #213252

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GIPR (GIPR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-N-Blast-ultraID

    Plasmid

    #207682

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. COM-epegRNA-Dnmt1

    Plasmid

    #211376

    Purpose

    Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    COM-epegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP VAMP8

    Plasmid

    #42311

    Depositor

    Thierry Galli

    Article

    Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

    Insert

    VAMP 8 (VAMP8 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

    Plasmid

    #112285

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…

    Promoter

    EF-1 alpha

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-CaaX(Hras)

    Plasmid

    #108886

    Purpose

    Expression of membrane localized mCherry

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    mCherry-CaaX(Hras)
    

    Use

    Can be used for mammalian expression or in vitro …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKS004 - pCAGGS-3XFLAG-CTCF-eGFP

    Plasmid

    #156438

    Purpose

    For transient expression of mouse CTCF tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse CTCF (Ctcf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmyc-GFP-TNRC6A

    Plasmid

    #41999

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. AK3L1

    Plasmid

    #39135

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AK3L1 (AK3 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pLKO.1 shRUNX1 puro

    Plasmid

    #45816

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    Runx1 (RUNX1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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