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Plasmid#22776PurposeRetroviral expression of tamoxifen-inducible Cre-ERT2DepositorInsertCreERT2
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-NHK-MYC-His6
Plasmid#185350PurposeAssessing of ubiquitination in cellsDepositorInserta1-antitrypsin Hong Kong Null mutant
UseTagsMyc, 6xHisExpressionMammalianMutationNHK mutantPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin
Plasmid#187956PurposeFKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-hHDAC4-SpdCas9-tagBFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-EGFP-ALDH1A1-Neo
Plasmid#189742PurposeThe construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.DepositorInsertALDH1A1 (ALDH1A1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterPGKAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Blast)
Plasmid#80921PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSIIhyg-RGECO1.0
Plasmid#175813PurposeThe lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.DepositorInsertRGECO1.0
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP-GFP-shch-TOG
Plasmid#69113PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationSilent mutations to confer shRNA resistance.PromoterCMVAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWJB9
Plasmid#126650PurposeExpresses AdrA from a modified pLAFR3DepositorInsertadrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
UseTags8xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-NCre
Plasmid#106368PurposeN-terminal part of the split-Cre SystemDepositorInsertNCre
UseCre/LoxTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
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EF1a_ESRRG_P2A_Hygro_Barcode
Plasmid#120435PurposeBarcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertESRRG (ESRRG Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
L304-EGFP-Tubulin-WT
Plasmid#64060PurposeEGFP fused at the N-terminus of tubulin; lentiviral constructDepositorInserttubulin
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Floxed-STOP Fluc
Plasmid#122962PurposeFirefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reactionDepositorInsertpcDNA3.1_Floxed-STOP Fluc
UseCre/Lox and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
GluN2A (px330:2-guideCas9)
Plasmid#169428PurposeExpression of Cas9 and 2 guidesDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Soma-jGCaMP8m
Plasmid#169257PurposeAAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.DepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertSoma-jGCaMP8m
UseAAVTags6xHis-tagExpressionMammalianMutationPromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
t1-435
Plasmid#166578PurposeFor expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertTln1 Head (1-435) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-435 onlyPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Banshee RunxDN mCherry
Plasmid#80158Purposeretroviral expression of Runx1DN and mCherryDepositorInsertRunx1 (Runx1 Mouse)
UseRetroviralTagsHA and IRES-mCherryExpressionMammalianMutationDN--truncated version of the Runx1 protein contai…PromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-AuroraAKD-C-7
Plasmid#54998PurposeLocalization: Centrosomes, Excitation: 587, Emission: 610DepositorInsertAuroraA KD (AURKA Human)
UseTagsmCherryExpressionMammalianMutationKinase Dead - I57V, K162RPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only