We narrowed to 7,632 results for: ALP

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FRB-polySH35

Plasmid

#175234

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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FRB-polySH33

Plasmid

#175233

Purpose

Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_GFP*_Intein_DD

Plasmid

#162812

Purpose

STELLA plasmid for amber suppression of C-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

GFP(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

GFP(Opal247,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate5

Plasmid

#164999

Purpose

Expression of candidate TCR #5 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pXZX353

Plasmid

#169457

Purpose

MATα (alpha) fragment in pTOPO vector

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

MatA

Expression

Bacterial

Promoter

lac

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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R-R334W-(Kin-7)

Plasmid

#28178

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Expression

Mammalian

Mutation

R334W

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pAS1_4xh7SKPylT_EF1_NoBody*_Intein_DD

Plasmid

#162813

Purpose

STELLA plasmid for amber suppression of C-terminally labelled NoBody

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

NoBody(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

NoBody(Opal69,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LI A-B EF1 c2

Plasmid

#54435

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

promoter region of A. thaliana EF1alpha1 /AT1G07940.1

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pDM_noro_S2_lacZA

Plasmid

#118816

Purpose

T7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch S2

Expression

Bacterial

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*NoBody

Plasmid

#162807

Purpose

STELLA plasmid for amber suppression of N-terminally labelled NoBody

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)NoBody

Use

Piggybac

Expression

Mammalian

Mutation

NoBody (Met1, Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate7

Plasmid

#165003

Purpose

Expression of candidate TCR #7 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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512 pME-miR

Plasmid

#26032

Depositor

Nathan Lawson

Article

Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

Insert

ef1a (eef1a1l1 Zebrafish)

Use

Entry vector

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only
pDM_noro_S6_lacZA

Plasmid

#118817

Purpose

T7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch S6

Expression

Bacterial

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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PALI1_pHIVEGFP

Plasmid

#191252

Purpose

Expresses PALI1 in mammalian cells

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LCoR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

NA

Promoter

EF1alpha

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PRKAA2ΔC

Plasmid

#30307

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α2 ΔC (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids Ser 527 to Arg 552

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only
pCoofy42

Plasmid

#55190

Purpose

Pichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…

Expression

Yeast

Promoter

AOX1

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24 shRescue

Plasmid

#28139

Depositor

Michelle Barton

Article

Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Mutation

A489V; aa 308 and 309 mutated for shRNA rescue

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pT2-cryR;basPEgfp

Plasmid

#90169

Purpose

contains a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

basalPEgfp

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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MLM3585

Plasmid

#43885

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: HD (binds C)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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pTU1-A-lacZ

Plasmid

#72935

Purpose

Level 1 Destination vector - Position A

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - A site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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