Addgene

We narrowed to 21,419 results for: ESC;

Showing: 8131 - 8160 of 21419 results

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pEGFP-C1-BBS18

Plasmid

#218718

Purpose

Expresses N-terminally EGFP-tagged BBS18 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS18

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-SFFVp-gI-BlastR

Plasmid

#207179

Purpose

Gateway compatible Lentiviral Expression Plasmid SFFVp-IRES-BlastR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFVp

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB605-GBSSI

Plasmid

#213867

Purpose

Binary vector for the expression of GBBSI fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

GRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB560-GBSSI

Plasmid

#213866

Purpose

Binary vector for the expression of GBBSI fused to the tagRFP in plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

GRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA5k-AcrAB-AsLOV2*(543)

Plasmid

#210863

Purpose

IPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.

Depositor

Mary Dunlop

Article

Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

Inserts

acrA
acrB

Use

Synthetic Biology

Tags

AsLOV2*(543)

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-yATOM_SH2-KDEL

Plasmid

#209714

Purpose

Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-yATOM_SH2-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_WDR5 (R80A)

Plasmid

#209713

Purpose

Mammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutation

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_WDR5 (R80A)

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL

Plasmid

#209718

Purpose

Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-NbGFP_miRFP670nano3-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-Clover-KDEL

Plasmid

#209720

Purpose

Mammalian expression of clover localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-Clover-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-yATOM_SH2

Plasmid

#209715

Purpose

Mammalian expression of y-ATOM biosensor for SH2 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-yATOM_SH2

Use

Tags

Cos4

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pWR95

Plasmid

#203916

Purpose

BLINCAR plasmid for gene deletion, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

modified MCS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SNIFP

Plasmid

#197210

Purpose

Expresses the protein of SNIFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

SNIFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmCardinal-T2A-HO1

Plasmid

#197249

Purpose

Expresses the protein of wmCardinal-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmCardinal-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP720-T2A-HO1

Plasmid

#197248

Purpose

Expresses the protein of wmiRFP720-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP720-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-pnRFP

Plasmid

#201678

Purpose

Bacteria protein expression of pnRFP biosensor

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

pnRFP

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAcBAC3-POLY-pnRFP

Plasmid

#201680

Purpose

Boost the expression of pnRFP in mammalian cells (e.g., macrophage RAW 264.7 cells)

Depositor

Huiwang Ai

Article

Pang et al ACS Chem Biol. 2023 Jun 16;18(6):1388-13

Insert

813

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-ef1a-DIO-eGCaMP(plus)

Plasmid

#201149

Purpose

AAV virus production for Cre recombinase dependent expression of eGCaMP(plus)

Depositor

Andre Berndt

Article

Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

Insert

eGCaMP+

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a no GFP)

Plasmid

#172339

Purpose

mnucTomato

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a)TetR

Plasmid

#172352

Purpose

Tet Repressor reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

TetR
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-NptII-9

Plasmid

#197744

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Neomycin phosphotransferase (NptII)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-HpaB-9

Plasmid

#197756

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

FBP: HpaB (caffeic acid biosynthesis)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-6-HpaC-9

Plasmid

#197757

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

FBP: HpaC (caffeic acid biosynthesis)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-mCBP80

Plasmid

#192313

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-mCBP80

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-mCBP80

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-IBB

Plasmid

#192314

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-IBB

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-IBB

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mIFP

Plasmid

#197214

Purpose

Expresses the protein of mIFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mIFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-EGFP

Plasmid

#196606

Purpose

Dual-Luciferase Reporter vector carrying an EGFP direct sequence

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSF11-wiRFP713-T2A-HO1

Plasmid

#197247

Purpose

Expresses the protein of wiRFP713-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wiRFP713-T2A-HO1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP713

Plasmid

#197209

Purpose

Expresses the protein of iRFP713 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP713

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.8

Plasmid

#197206

Purpose

Expresses the protein of BDFP1.8 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.8

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iRFP682

Plasmid

#197204

Purpose

Expresses the protein of iRFP682 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP682

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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