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pMT-GFP-p150Dynactin

Plasmid

#24283

Depositor

Ron Vale

Article

Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

Insert

p150 Dynactin (DCTN1-p150 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
1352wUV2 F1bbs kan GCGTGGNNN

Plasmid

#18753

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

3 tandem zinc fingers

Use

Tags

Expression

Bacterial

Mutation

A Kan cassette (1090bp) is inserted between two b…

Promoter

Available since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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FKBP-YPet-RanGAP*

Plasmid

#175245

Purpose

Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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CyPet-FKBP-E2

Plasmid

#175244

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-PTEN D92A

Plasmid

#50521

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

D92A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z25

Plasmid

#120068

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCytERM-EBFP2

Plasmid

#184939

Purpose

ER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-EBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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SGL40C.EFS.RFP657

Plasmid

#69147

Purpose

Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

sgRNA
EFS
tagRFP657

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab4 DN

Plasmid

#37691

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab4 (Rab4 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S22N (dominant negative)

Promoter

GAL4

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pNTPTP alpha (S180A/S204A)

Plasmid

#17700

Depositor

Xinmin Zheng

Article

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S180A S204A (PTPRA Human)

Use

Tags

Expression

Mammalian

Mutation

S180A / S204A

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pMaCTag-P19

Plasmid

#124789

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Expression

Mutation

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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2674 pBS Bcl-2 P1+P2

Plasmid

#8746

Depositor

Stanley Korsmeyer

Article

Young et al Mol Cell Biol 1993 Jun;13(6):3686-97.

Insert

Bcl-2 P1+P2 (BCL2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pCsy-crRNA-EV

Plasmid

#153944

Purpose

For cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pPyCAG-NE-EGFP-IRES-Pac

Plasmid

#183617

Purpose

Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

Insert

EGFP

Use

Tags

Human EMD transmembrane domain and NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CMV10-Flag-mMcl-1 KR

Plasmid

#32979

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

All lys changed to Arg

Promoter

CMV

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

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pBBK75 pHomL-RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

Plasmid

#179059

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-2xPH(PLCd), a marker for the plasma membrane

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT sfGFP 150TAG

Plasmid

#154771

Purpose

plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-AtAFB2-mTagBFP2

Plasmid

#179889

Purpose

Expresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

AtAFB2 (AFB2 Mustard Weed, Synthetic)

Use

Lentiviral

Tags

mTagBFP2

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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p413GAL1-Zipp.-L-IFP-F[2] (Yeast)

Plasmid

#52893

Purpose

Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Zipp.-L-IFP-F[2]

Use

Tags

L-IFP-F[2]

Expression

Yeast

Mutation

Promoter

GAL1

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pFastBac1-OSF-TRIM5alpha(rhesus)

Plasmid

#79033

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(rhesus)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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