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E1b-GFP-Tol2-Gateway dre SE-zic2a P

Plasmid

#89385

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDH5

Plasmid

#83775

Purpose

Useful for tagging yeast proteins with C-terminal YFP tag; his selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP

Tags

YFP

Expression

Yeast

Mutation

S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only

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4QPP

Plasmid

#61964

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HRMT1L6:PBC001-A12:C213642 (PRMT6 Human)

Use

Baculovirus expression

Tags

6x His and TEV cleavage site

Mutation

See comments

Promoter

Polyhedrin

Available Since

Sept. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits
4RG2

Plasmid

#64658

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

53BP1 (TP53BP1 Human)

Tags

6xHis-TEV and MHHHHHHSSGRENLYFQG

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available Since

April 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
psbA2-Ptrc-T7-g10-PHLS (e)

Plasmid

#52310

Purpose

Replaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc-T7

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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KHBD00551

Plasmid

#39646

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1849 (run Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(1234-1443)

Plasmid

#35078

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Mutation

Contains aa 1234-1443

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00298

Plasmid

#34424

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG4029 (jumu Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00158

Plasmid

#34312

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG33183 (Hr46 Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
DPY-pNCS

Plasmid

#17365

Depositor

Stephen Goff

Article

Yuan et al J Virol. 2000 Aug . 74(16):7250-60.

Insert

MMLV proviral DNA

Mutation

deletion that removes 5 amino acids (DPPPY) from …

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pBABE-zeo small T C97S E102Q

Plasmid

#9064

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 97, 102 (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Mutation

C97S, E102Q

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
PB-Ef1a-RPS27A-UTR-PP7-WPRE

Plasmid

#198339

Purpose

PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

RPS27A-3'UTR-PP7 stem loops x24

Use

Piggybac

Tags

PP7 stem loops

Expression

Mammalian

Promoter

Ef1a

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET30b jGCaMP8f F366H

Plasmid

#204139

Purpose

Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Phenylalanine 366 changed to histidine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-NOG(CmYLCV)

Plasmid

#202014

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

dCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1

Tags

GB1 and sfGFP

Expression

Plant

Promoter

CmYLCV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_D-CmYLCV-NbGP41P-sfGFP-VP64-GB1

Plasmid

#202010

Purpose

Moclo level 1 - Module D, Promoter: CmYLCV, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: ZmUBI

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Insert

NbGP41P-sfGFP-VP64-GB1

Use

Synthetic Biology; Moclo level 1 vector

Tags

GB1 and sfGFP

Promoter

CmYLCV

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_DRD1-NTEV-TCS-GV-2xHA

Plasmid

#194358

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

DRD1-NTEV-TCS-GV-2xHA (DRD1 Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

Plasmid

#184335

Purpose

Expression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

Nrxn3-alpha-GPI (Nrxn3 Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Paragranulin+linker 1

Plasmid

#176915

Purpose

express Paragranulin with linker 1 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Paragranulin + linker 1 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pJV136

Plasmid

#124255

Purpose

Lentiviral vector (pRRL) containing KRAS G12V specific T cell receptor, clone 9

Depositor

Stanley Riddell

Article

Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

Insert

T cell receptor (KRAS Human)

Use

Lentiviral

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

Plasmid

#160571

Purpose

Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNAscaffold 2.1 scRNA

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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