We narrowed to 9,184 results for: Pol;

1. W118-1_hTGM5-V5

   Plasmid

   #180414

   Purpose

   lentiviral transduction of human TGM5 (with a C-terminal V5 tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM5 (TGM5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDT386

   Plasmid

   #174733

   Purpose

   Expresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.

   Depositor

   Jukka Finne

   Article

   Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

   Insert

   DT386
   

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Deleted amino acids 412-560 (the receptor binding…

   Promoter

   T5

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX4T3-GST-EGFP

   Plasmid

   #177136

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   EGFP
   

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-puro-mycRSK2wt

   Plasmid

   #15827

   Depositor

   Jing Chen

   Article

   Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

   Insert

   Ribosomal S6 kinase 2 (Rps6ka3 Mouse)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   wild type

   Available Since

   Nov. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-Tagger

   Plasmid

   #129396

   Purpose

   Expresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoter

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS Biol. 2019 Aug 8;17(8):e3000374. do

   Insert

   rpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
   

   Tags

   FLAG, HA, and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCWXPG-UBI-MafA

   Plasmid

   #114302

   Purpose

   Constitutive expression of the MafA trancription factor

   Depositor

   Patrick Salmon

   Article

   Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

   Insert

   MafA (MAFA Human)
   

   Use

   Lentiviral

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScalps_puro_mIL-2Rα

   Plasmid

   #59917

   Purpose

   Expresses mouse interleukin-2 receptor alpha chain

   Depositor

   Silvia Monticelli

   Article

   Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

   Insert

   interleukin 2 receptor alpha (Il2ra Mouse)
   

   Use

   Lentiviral

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. AAV-shRNA_Tet3

   Plasmid

   #85740

   Purpose

   shRNA against mouse Tet3

   Depositor

   Hongjun Song

   Article

   Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

   Insert

   Tet3 (Tet3 Mouse)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCALPs-HA-NFAT1 (4-460)-GFP

   Plasmid

   #88879

   Purpose

   Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

   Insert

   HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
   

   Use

   Lentiviral

   Tags

   GFP fusion protein and HA tag

   Expression

   Mammalian

   Mutation

   amino acids 4-460 only; L78P & L287P *see bel…

   Promoter

   SFFV (spleen focus forming virus)

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGM8

    Plasmid

    #220166

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    TSPO (TSPO Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-OF (mutant TCF-4 sites)

    Plasmid

    #16559

    Depositor

    Bert Vogelstein

    Article

    Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

    Insert

    TCF-4 binding sites (TCF4 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    3 copies of mutant Tcf-4 binding sites

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIA1385

    Plasmid

    #166862

    Purpose

    Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX Flag RagC S75N

    Plasmid

    #112757

    Purpose

    express Flag-RagC (S75N mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (S75N) (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    S75N

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTon1(acgfp1)

    Plasmid

    #233648

    Purpose

    Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.

    Depositor

    Takeo Kubo

    Article

    Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10

    Insert

    target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
    

    Use

    Amphibian expression, tet-on

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. hsp70: mKate2-DN Kif5a

    Plasmid

    #105972

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    Kif5a (kif5aa Zebrafish)
    

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    truncation of aa416-1033

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZDonor-Nm-Cas9-gRNA-hU6

    Plasmid

    #61366

    Purpose

    empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDECKO-mCherry GFP

    Plasmid

    #78535

    Purpose

    Control plasmid (paired gRNAs against GFP)

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET14b-dabAB2

    Plasmid

    #133013

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. cyclin D1-HA-pBabe-Hygro

    Plasmid

    #22703

    Depositor

    William Kaelin

    Article

    Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

    Insert

    cyclin D1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG32-3HA-3FLAG

    Plasmid

    #154081

    Purpose

    For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

    Depositor

    Cornelia Kilchert

    Article

    Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

    Insert

    3xHA-3xFLAG-hygR
    

    Use

    Pcr-based tagging in yeast

    Tags

    3xHA-3xFLAG

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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