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We narrowed to 6,844 results for: tet

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Showing: 5461 - 5480 of 6844 results

  4. Desmoplakin I affinity mutant (F40-based tension sensor)

    Plasmid
    #119190
    Purpose
    The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119190-plasmid-map-sequence-id-232293

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  5. circRAJ31-Toehold_mutant

    Plasmid
    #69930
    Purpose
    Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.
    Depositor
    Alfonso Jaramillo
    Article
    Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do
    Insert
    plTet-circRAJ31_toeholdmutant
    Use
    Synthetic Biology
    Mutation
    4 bases mutated in toehold of riboregulator
    Promoter
    PltetO1-2
    Available Since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    69930-plasmid-map-sequence-id-124217

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  6. pYCTK028

    Plasmid
    #176732
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Tetrapisispora phaffii (receiver part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    ste2Tp
    Use
    Synthetic Biology
    Mutation
    Codon-optimized for expression in S. cerevisiae
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176732-plasmid-map-sequence-id-351572

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  7. pCL28-decoder

    Plasmid
    #159450
    Purpose
    Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    PlexAtet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    PlexAtet
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159450-plasmid-map-sequence-id-313198

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  11. pTS2163

    Plasmid
    #169632
    Purpose
    Tier-3 vector for CRISPR-mediated stable integration into the AAVS1 locus of the tetON-system (OTetO7-PCMVmin-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::A3-pA)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    OTetO7-PCMVmin-SEAP-iRFP670::PmPGK1-rtTA-pA::A3
    Expression
    Mammalian
    Promoter
    tetO7-PhCMVmin / PPGK
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169632-plasmid-map-sequence-id-366785

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  12. JA431

    Plasmid
    #49937
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD KL-2 (KLF4 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pRAN357

    Plasmid
    #120898
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.
    Insert
    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    Expression
    Bacterial
    Promoter
    Ptet
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120898-plasmid-map-sequence-id-235310

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  14. JA1290

    Plasmid
    #49968
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-9 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. JA1261

    Plasmid
    #49963
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-6 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. JA1252

    Plasmid
    #49956
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-3 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. MLM3733

    Plasmid
    #49964
    Purpose
    Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CD HB-6 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. Equalizer-H plasmid with onboard mCherry cassette

    Plasmid
    #169733
    Purpose
    Equalizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    tetR-P2A-eGFP-miR(FF3) target-miR(FF3)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-tetO2
    Available Since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169733-plasmid-map-sequence-id-334140

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  19. pDUP1

    Plasmid
    #223516
    Purpose
    inactive cogfp under control of Ptet, cogfp under control of Ptac
    Depositor
    Yolanda Schaerli
    Article
    Mihajlovic et al eLife. 2025 Apr 2;13:RP97216. doi: 10.75
    Inserts
    CoGFP
    CoGFP inactivated
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    anti-dimerization mutations S129G, 22 C154S, D156…
    Promoter
    Ptac, Ptac and Ptet, and Ptet
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    223516-plasmid-map-sequence-id-444180

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Showing: 5461 - 5480 of 6844 results