Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 70,882 results for: LAS

Showing: 8221 - 8250 of 70882 results

  1. hSyn-DIO-somBiPOLES-eGFP

    Plasmid
    #192584
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192584-plasmid-map-sequence-id-379290

    Enlarge

  2. IRES-creERT2-FNF

    Plasmid
    #188230
    Purpose
    EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette
    Depositor
    Mario Capecchi
    Insert
    IRES-creERT2-FNF
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    188230-plasmid-map-sequence-id-371215

    Enlarge

  3. AnkyrinB-GFP/pEGFP-N1

    Plasmid
    #197327
    Purpose
    Plasmid expressing an antigen targeting AnkyrinB
    Depositor
    Vann Bennett
    Insert
    AnkyrinB (Ank2 Rat)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    197327-plasmid-map-sequence-id-406733

    Enlarge

  4. O-TREM2-2

    Plasmid
    #166545
    Purpose
    scFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166545-plasmid-map-sequence-id-329237

    Enlarge

  6. p406ADH1

    Plasmid
    #15974
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    ADH1 promoter + polylinker + CYC1 terminator (ADH1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    ADH1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15974-plasmid-map-sequence-id-8459

    Enlarge

  7. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid
    #26976
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV5, AAV8, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26976-plasmid-map-sequence-id-313556

    Enlarge

  8. pCW57.1 CCNE1

    Plasmid
    #164144
    Purpose
    Doxycycline inducible expression of Cyclin E1
    Depositor
    Richard Possemato
    Insert
    CCNE1 (CCNE1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    164144-plasmid-map-sequence-id-325861

    Enlarge

  9. p406TEF1

    Plasmid
    #15976
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15976-plasmid-map-sequence-id-8461

    Enlarge

  10. pAAV-phSyn-flex-SypEGFP

    Plasmid
    #153203
    Purpose
    Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter
    Depositor
    Lynette Lim
    Insert
    synaptophysin-EGFP
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    rat synapsin
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153203-plasmid-map-sequence-id-298916

    Enlarge

  11. pGBW-m4134906

    Plasmid
    #151960
    Purpose
    Mammalian expression plasmid for Human coronavirus OC43 nucleocapsid
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    Human coronavirus OC43 nucleocapsid protein (EYW02_gp8 Human coronavirus OC43)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151960-plasmid-map-sequence-id-306272

    Enlarge

  12. pcDNA3.2-V5-DEST-hSFRP1-his

    Plasmid
    #189540
    Purpose
    Protein expression of SFRP1 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    his
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    189540-plasmid-map-sequence-id-374109

    Enlarge

  13. p406GAL1

    Plasmid
    #17437
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GAL1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    March 12, 2008
    Availability
    Academic Institutions and Nonprofits only
    17437-plasmid-map-sequence-id-8611

    Enlarge

  14. pN3-Nipbl

    Plasmid
    #107716
    Purpose
    Expression plasmid for 3xFLAG-tagged NIPBL
    Depositor
    Guntram Suske
    Insert
    NIPBL (Nipbl Mouse)
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    107716-plasmid-map-sequence-id-307684

    Enlarge

  15. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

    Plasmid
    #100054
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV1, AAV2, AAV5, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R mutation in ChR2
    Promoter
    CAG
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100054-plasmid-map-sequence-id-183171

    Enlarge

  16. p404TEF1

    Plasmid
    #15972
    Depositor
    Fred Cross, Nicolas Buchler
    Insert
    TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    TEF1 promoter is between SacI and XbaI restrictio…
    Promoter
    Available since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15972-plasmid-map-sequence-id-8457

    Enlarge

  17. pAAV-synp-F-synaptophysin-GCaMP6f

    Plasmid
    #102996
    Purpose
    Expresses synaptophysin fused GCaMP6f under the synapsin promoter
    Depositor
    David Cox
    Insert
    Synaptophysin-GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    102996-plasmid-map-sequence-id-218069

    Enlarge

  18. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83475-plasmid-map-sequence-id-358352

    Enlarge

  19. EF1α-mNeonGreen-Z1

    Plasmid
    #135613
    Purpose
    mNeonGreen monomeric fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    mNeonGreen
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-HTLV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135613-plasmid-map-sequence-id-269406

    Enlarge

  20. pSCM167-Flag-hSFRP1

    Plasmid
    #189538
    Purpose
    Protein expression of SFRP1 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    189538-plasmid-map-sequence-id-374368

    Enlarge

  21. pET28a-HIV p24

    Plasmid
    #212195
    Purpose
    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus
    Depositor
    Richard C. Willson
    Insert
    Chain A, Capsid protein p24, HIV type 1
    Use
    Tags
    Polyhistidine
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212195-plasmid-map-sequence-id-430368

    Enlarge

  22. pGFP FAK Y397F

    Plasmid
    #50516
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    protein tyrosine kinase, mutated
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Y397F
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  24. pCT02

    Plasmid
    #212152
    Purpose
    Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.
    Depositor
    Kang Zhou
    Insert
    FAST-PETase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S121E, D186H, R224Q, N233K, R280A
    Promoter
    PT7
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212152-plasmid-map-sequence-id-424774

    Enlarge

  25. p14-9VN

    Plasmid
    #50930
    Purpose
    Expresses human SRP14 and SRP9 proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Use
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50930-plasmid-map-sequence-id-422738

    Enlarge

  26. pCMVβ-p300.DY-myc

    Plasmid
    #30490
    Depositor
    Tso-Pang Yao
    Insert
    p300 (EP300 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    aspartic acid 1399 converted to tyrosine (Acetyla…
    Promoter
    Available since
    Oct. 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  27. FUG-P2A-tC3-W

    Plasmid
    #129475
    Purpose
    3rd-generation lentiviral vector for hUbC-driven co-expression of EGFP and truncated C3
    Depositor
    Robert Gross
    Insert
    truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human ubiquitin C
    Available since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    129475-plasmid-map-sequence-id-384588

    Enlarge

  28. pGBW-m4134910

    Plasmid
    #151939
    Purpose
    Mammalian expression plasmid for Human coronavirus NL63 nucleocapsid
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    Human coronavirus NL63 nucleocapsid protein (HCNV63gp6 Human coronavirus NL63)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151939-plasmid-map-sequence-id-306268

    Enlarge

  29. pDON-AI-Npm2(N533)

    Plasmid
    #51475
    Purpose
    Express mouse phosphorylation-mimicking Npm2 (p-Npm, N533)
    Depositor
    Shunsuke Ishii
    Insert
    Nucleoplasmin 2 (Npm2 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Eleven codons (5, 6, 7, 8, 10, 82, 95, 104, 184, …
    Promoter
    Available since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51475-plasmid-map-sequence-id-78547

    Enlarge

  30. pML104-NatMx3

    Plasmid
    #83477
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    NatMx3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 8221 - 8250 of 70882 results