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pPBT-peRNA_GG-Puro

Plasmid

#173220

Purpose

piggyBac transposon containing hU6 promotor and pegRNA cloning site

Depositor

Jacob Giehm Mikkelsen

Article

Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

Inserts

pegRNA cloning cassette
PuroR

Use

Piggybac

Expression

Mammalian

Promoter

EF1-a and hU6

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE135

Plasmid

#118489

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-16

Plasmid

#160716

Purpose

T-nos Terminator bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

nopaline synthase terminator (T-nos) bordered by BsaI restriction sites

Use

Synthetic Biology

Mutation

Incorporation of BsaI restriction sites

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB2-B/BRISBANE/60/2008

Plasmid

#187917

Purpose

Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 2

Tags

UNTAGGED

Promoter

CMV Promoter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Hic1 GFP

Plasmid

#106953

Purpose

Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

anti-Hic1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE137

Plasmid

#118491

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PI4KAC1001(D1957A)

Plasmid

#139312

Purpose

Recruitable PI4KA kinase-dead catalytic fragment

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)

Expression

Mammalian

Mutation

D1957A, G1986D

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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pHsp83-NLS-HA-2xPCP-2xGFP

Plasmid

#71243

Purpose

Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

2xPCP-2xGFP

Use

P element-mediated germline transformation

Tags

EGFP

Expression

Insect

Promoter

Hsp83

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMRE130

Plasmid

#118484

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE133

Plasmid

#118487

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-fPV-GFP

Plasmid

#22914

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu parvalbumin promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pMRE162

Plasmid

#118510

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PI4KAC1001

Plasmid

#139311

Purpose

Recruitable PI4KA catalytic fragment

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)

Expression

Mammalian

Mutation

G1986D

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

Plasmid

#187922

Purpose

Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Tags

3xFLAG

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-RAB5A

Plasmid

#176488

Purpose

For lentiviral transduction of 3FLAG-RAB5A

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

RAB5A (RAB5A Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEC3006 (pSpy1C-PZn_ffluc)

Plasmid

#218515

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of Plac_mrfp cassette

Promoter

PZn (PczcD)

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE152

Plasmid

#118502

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSF-p15ABAD- YFP

Plasmid

#186571

Purpose

p15A ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

BAD promoter

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE165

Plasmid

#118513

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-TdTomato-WPRE-pA

Plasmid

#37092

Purpose

Expresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

TdTomato

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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