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pDONR223_EGFR_p.774_775insHV

Plasmid

#82852

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway Cloning

Mutation

HV insertion between AA 774 and 775

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SAMHD1_p.D218H

Plasmid

#81513

Purpose

Gateway Donor vector containing SAMHD1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SAMHD1 (SAMHD1 Human)

Use

Gateway Cloning

Mutation

D218H; G404E; A546V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KEAP1_p.G524C

Plasmid

#82779

Purpose

Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KEAP1 (KEAP1 Human)

Use

Gateway Cloning

Mutation

G524C; C624S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.Q61L

Plasmid

#81302

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway Cloning

Mutation

Q61L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHOA_p.R5Q

Plasmid

#81294

Purpose

Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHOA (RHOA Human)

Use

Gateway Cloning

Mutation

R5Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_H2AFX_p.K134N

Plasmid

#81275

Purpose

Gateway Donor vector containing H2AFX , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

H2AFX (H2AX Human)

Use

Gateway Cloning

Mutation

K134N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.G12C

Plasmid

#81266

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway Cloning

Mutation

G12C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR-27a-3p

Plasmid

#82438

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-27a-3p (MIR27A Human)

Mutation

Three copies hsa-miR-27a-3p Complementary region …

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,4A5C6G7G-GFP

Plasmid

#80914

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P4A5C6G7G and PM,4A5C6G7G

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-P/PM,5C6A-GFP

Plasmid

#80910

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P5C6A and PM,5C6A

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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HcKan_P

Plasmid

#65314

Purpose

receiving vector of promoters for the construction of standard promoter parts in YeastFab work

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRVdG-mCherry-N-P-M-EGFP-L

Plasmid

#52480

Purpose

Expresses mCherry and EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mCherry
EGFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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40 p tie2

Plasmid

#13064

Depositor

Nathan Lawson

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

tie2 (tek Zebrafish)

Mutation

Q817E (please see depositor's comment below)

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
p-miR-Report + WT miR-181 BS in CDX2 3UTR

Plasmid

#22792

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Wild-type miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)

Use

Luciferase

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pLINK cyclin T1 (1-280) myc (K93D) (P#580)

Plasmid

#14611

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-280) K93D (CCNT1 Human)

Tags

myc

Expression

Mammalian

Mutation

K93D. Deficient in binding to CDK9. Inactive in 3…

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pLINK CDK9 myc (D167N) (P#1084)

Plasmid

#14637

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Tags

myc

Expression

Mammalian

Mutation

D167N. Kinase negative mutant.

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pAAV.CAG.iGluSnFR3.v857.SGZ

Plasmid

#178334

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZ

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.SGZ

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

Expression

Mammalian

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-hCCND1-HA

Plasmid

#174154

Purpose

Lentiviral vector expressing HA-tagged human cyclin D1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

hemagglutinin (HA)

Expression

Mammalian

Promoter

EF1A

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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YA1532: pAAV_ CAG-FLEX-paQuasAr3

Plasmid

#107702

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

paQuasAr3

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

Plasmid

#92121

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Article

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-pj23119

Use

CRISPR

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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