We narrowed to 6,088 results for: pcDNA 3.1

1. VN-Sph1

   Plasmid

   #89755

   Purpose

   expresses human Synphilin fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Dominguez-Meijide et al Neurobiol Dis. 2021 Apr;151:105256. doi:

   Insert

   SNCAIP (SNCAIP Human)
   

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-MDFI

   Plasmid

   #207836

   Purpose

   Tagged MDFI for western blotting and imaging

   Depositor

   Charles Cox

   Article

   Zhou et al Science. 2023 Aug 18;381(6659):799-804.

   Insert

   MDFI (MDFI Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. cyt-GafD-mTurboID-V5

   Plasmid

   #184640

   Purpose

   Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

   Depositor

   Charlie Fehl

   Article

   Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

   Insert

   cyt-GlycoID-V5
   

   Tags

   Nuclear export sequence and V5 tag

   Expression

   Mammalian

   Mutation

   GafD -> expressed aa22-178 ; BirA* -> expre…

   Promoter

   CMV

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYFP-ErbB2

   Plasmid

   #66948

   Purpose

   Mammalian expression of rat ErbB2 tagged with YFP

   Depositor

   Mads Lerdrup, Bo van_Deurs

   Article

   Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

   Insert

   ErbB2 (Erbb2 Rat)
   

   Tags

   6xHis, V5 Tag, and YFP

   Expression

   Mammalian

   Mutation

   (See depositor comment below on mutations A24T an…

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. Arrb1-RFP

   Plasmid

   #35401

   Depositor

   Robert Lefkowitz

   Article

   Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

   Insert

   beta-arrestin-1 (Arrb1 Rat)
   

   Tags

   RFP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

6. FLAG-MED22

   Plasmid

   #15423

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED22 (MED22 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

7. LUC01 (L/Sec/P)

   Plasmid

   #112144

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
   

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U)

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. β₂AR-mCFP

   Plasmid

   #38260

   Depositor

   Robert Lefkowitz

   Article

   Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

   Insert

   β₂AR (ADRB2 Human)
   

   Tags

   Flag, mCFP, and signal peptide sequence (MKTIIALS…

   Expression

   Mammalian

   Mutation

   diglycine linker (replacing terminator codon)

   Promoter

   CMV

   Available Since

   Aug. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

9. nuc-GafD-mTurboID-HA

   Plasmid

   #184641

   Purpose

   Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

   Depositor

   Charlie Fehl

   Article

   Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

   Insert

   nuc-GlycoID-HA
   

   Tags

   3xHA tag and Nuclear localization sequence

   Expression

   Mammalian

   Mutation

   GafD -> expressed 22-178 ; BirA* -> express…

   Promoter

   CMV

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-MED18

    Plasmid

    #15419

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED18 (MED18 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

11. MLF2-GFP-3xFLAG

    Plasmid

    #230986

    Purpose

    Expresses GFP tagged MLF2 in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

    Insert

    MLF2 (MLF2 Human)
    

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. NSP4-WT-Flag

    Plasmid

    #210339

    Purpose

    Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. LUC05 (L/Cys/P)

    Plasmid

    #112146

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

    Insert

    Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Perox-mTurq2-Apollo-NADP+

    Plasmid

    #193304

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Tags

    mTurqoise2, peroxisomal targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. CoV2-N-B.1.1.7

    Plasmid

    #177958

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    

    Expression

    Mammalian

    Mutation

    D3L,R203K,G204R,S235F

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Mito-mTurq2-Apollo-NADP+

    Plasmid

    #193303

    Purpose

    Expresses mTurq2-Apollo-NADP+ sensor localized to the mitochondria

    Depositor

    Jonathan Rocheleau

    Article

    Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

    Insert

    G6PD (G6PD Human)
    

    Tags

    mTurqoise2 and mitochondrial targeting sequence

    Expression

    Mammalian

    Mutation

    S40A, R72Q, H201N, K205T

    Promoter

    T7

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SpyTag-RBD

    Plasmid

    #159999

    Purpose

    Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CCM2_wt_eGFP

    Plasmid

    #208876

    Purpose

    Express eGFP-CCM2 in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT sequence

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-MED7

    Plasmid

    #15416

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED7 (MED7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. HiBiT-FZD10

    Plasmid

    #195848

    Purpose

    Expresses human FZD10 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD10 (FZD10 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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