We narrowed to 18,395 results for: Tec

1. pAAV-GfaABC1D-GRAB_eCB2.0

   Plasmid

   #164609

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S383T

   Promoter

   GfaABC1D

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFEndoNA

   Plasmid

   #174724

   Purpose

   Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

   Depositor

   Jukka Finne

   Article

   Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

   Insert

   GFP-endoNA fusion protein
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGWB543

   Plasmid

   #74877

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA011

   Plasmid

   #215973

   Purpose

   Fragmid fragment: (N' terminus) for C>T base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA010

   Plasmid

   #216038

   Purpose

   Fragmid fragment: (C' terminus) for C>T base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   UGI_v1.1; NpNLS_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA013

   Plasmid

   #215975

   Purpose

   Fragmid fragment: (N' terminus) for A>G base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB408

   Plasmid

   #74802

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGK112

   Plasmid

   #228127

   Purpose

   Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL2669 (pTnsABf_Vch)

   Plasmid

   #200897

   Purpose

   Expresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchTnsA-TnsB
   

   Use

   Tags

   Internal NLS between TnsA-TnsB fusion

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMV_AA088

    Plasmid

    #216104

    Purpose

    Knockout, EF1a-driven Cas9 (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF1alpha-rtTA-Antares

    Plasmid

    #197587

    Purpose

    Expresses TET-On reverse transactivator labeled with Antares

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    rtTA-P2A-Antares
    

    Use

    Tags

    Antares

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE3G-iRFP-B1-timestamp_A

    Plasmid

    #159924

    Purpose

    Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    iRFP-Timestamp A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS1051-Tier1-PhCMV-4xCox8

    Plasmid

    #169490

    Purpose

    Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pY027

    Plasmid

    #84742

    Purpose

    Expresses huLbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huLbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-Sp-gRNA-ALB_DF_B358c_attB

    Plasmid

    #182146

    Purpose

    To insert attB attachment site at ALB in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    ALB_B358c_attB pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-Sp-gRNA-ALB_DF_A277c_attB

    Plasmid

    #182145

    Purpose

    To insert attB attachment site at ALB in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    Alb_A277c_attB pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-ExRai-AktAR(T/A)

    Plasmid

    #118463

    Purpose

    Negative-control mutant for ExRai-AktAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LbABE8e(TadA-8e V106W)

    Plasmid

    #138508

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e V106W)-dLbCas12a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TadA-8e V106W

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBB75-ncRNA

    Plasmid

    #194087

    Purpose

    Expresses Ec86 ncRNA in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 ncRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-RAB-EKARev(T/A)

    Plasmid

    #118475

    Purpose

    Negative-control mutant for RAB-EKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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