We narrowed to 23,735 results for: fus
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pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Lhx3
Plasmid#32927DepositorAvailable SinceNov. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Ngn2
Plasmid#32928PurposeRetroviral expression of mouse Ngn2DepositorAvailable SinceNov. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA
Plasmid#86986PurposeFor cloning and expression of sgRNA together with expression of a Cas9-Venus fusion proteinDepositorInsertCas9-venus
TagsCas9-Venus fusion proteinExpressionMammalianPromoterCAGAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Isl1
Plasmid#32929DepositorAvailable SinceNov. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD63-(TEV)-tTA
Plasmid#162597Purposehuman CD63-TEV cleavage site-tTADepositorInserthuman CD63 (CD63 Human)
TagsTEV protease cleavage site and tTAExpressionMammalianPromoterCMV promoterAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P4:gs:nls:VPR
Plasmid#135983PurposeExpresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cellsDepositorInsertP4 coiled-coil fused to the VPR activation domain
ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-KRasCT
Plasmid#175835PurposeExpress mCherry with plasma membrane-targeting sequence in mammalian cellsDepositorInsertmCherry with plasma membrane-targeting sequence
TagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-dALFANb(3mut)-mGreenLantern
Plasmid#219662PurposeGal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLanternDepositorInsertDestabilised ALFA nanobody (3 mutations) fused to mGreenLantern
ExpressionInsectAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB_NSlmb-vhhGFP4
Plasmid#35577DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
ExpressionInsectAvailable SinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DD-T6B-MUT-YFP
Plasmid#235144PurposeExpresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoterDepositorInsertDHFR-fused mutant T6B peptide
UseAAVMutation5 tryptophan residues of T6B-WT mutatedPromoterhSynAvailable SinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMK365 (Tet-OsTIR1-loxP-PURO-loxP)
Plasmid#121185PurposeOsTIR1 introductionDepositorInsertTet-OsTIR1-loxP-PURO-loxP
UseCRISPRMutationOsTIR1 codon optimized for human cellsAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBK-EQR-BE3
Plasmid#85172PurposeExpresses EQR-BE3 in mammalian cellsDepositorInsertEQR-BE3
ExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSYN-PC5-003
Plasmid#42501DepositorAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFX-mito-eqFP650
Plasmid#174179PurposeExpress eqFP650 with mitochondria-targeting sequence in mammalian cellsDepositorInserteqFP650 with mitochondria-targeting sequence
Tagsthe mitochondria-targeting sequence of the cytoch…ExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-mKate2-Rab11
Plasmid#174463PurposeExpress ｍKate2 with recycling endosome-targeting sequence in mammalian cellsDepositorInsertmKate2 with recycling endosome-targeting sequence
Tagssmall GTPase (Rab11)ExpressionMammalianPromoterCMVAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate2-KDEL-pA
Plasmid#109546PurposeMultisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)DepositorInsertmKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pV92.2
Plasmid#78409PurposepKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron constructDepositorInsertsRT-Cas1
Cas2
Available SinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nrxn1beta AS4(-)-Fc
Plasmid#59313PurposeExpression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]DepositorInsertNrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
TagsFc (from human IgG)ExpressionMammalianPromoterCMV, T7Available SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-tTA
Plasmid#162598Purposehuman CD81-TEV cleavage site-tTADepositorInserthuman CD81 (CD81 Human)
TagsTEV protease cleavage site and tTAExpressionMammalianPromoterCMV promoterAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-EGFP-Sec61β
Plasmid#174466PurposeExpress EGFP with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertSirius with endoplasmic reticulum-targeting sequence
TagsSec61 subunit betaExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest-303
Plasmid#159678PurposeGateway destination vector for mammalian protein expressionDepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mGFP-LLO-mPMEL
Plasmid#174608Purposeexpresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitopeDepositorInsertmembrane GFP fused to LLO and mouse PMEL antigen
ExpressionMammalianAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-amylase
Plasmid#166859PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.DepositorInsertcsgBACEFG, with α-amylase fused to CsgA via flexible linker
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL2669 (pTnsABf_Vch)
Plasmid#200897PurposeExpresses human codon-optimized V. cholerae TnsA-TnsB fusion from a CMV promoter.DepositorInsertVchTnsA-TnsB
TagsInternal NLS between TnsA-TnsB fusionExpressionMammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFX-mito-roGFP
Plasmid#174181PurposeExpress roGFP with mitochondria-targeting sequence in mammalian cellsDepositorInsertroGFP with mitchondria-targeting sequence
Tagsthe mitochondria-targeting sequence of the cytoch…ExpressionMammalianPromoterCMVAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc:P4:gs:nls:ABI
Plasmid#135985PurposeExpresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cellsDepositorInsertP4 coiled-coil fused to the ABI domain
TagsMycExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST
Plasmid#175019PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertD/bovine/Nebraska/9-5/2012
TagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nrxn1beta AS4(+)-Fc
Plasmid#59314PurposeExpression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]DepositorInsertNrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
TagsFc (from human IgG)ExpressionMammalianPromoterCMV, T7Available SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only
