We narrowed to 10,134 results for: ada

1. pMKO.1-shCcnb2

   Plasmid

   #162794

   Purpose

   Ccnb2 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Ccnb2 shRNA (Ccnb2 Mouse)
   

   Use

   RNAi and Retroviral

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2R3a-3AT

   Plasmid

   #71273

   Purpose

   Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
   

   Use

   Gateway

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. NSK026 CMV-TO-p53R248W (FLP-IN)

   Plasmid

   #191168

   Purpose

   Expression of human p53 mutant R248W in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   p53(R248W) (TP53 Human)
   

   Expression

   Mammalian

   Mutation

   R248W

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1 MP1 shRNA

   Plasmid

   #26632

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   MP1 shRNA (LAMTOR3 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Nov. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pSW648 - pREV>>

   Plasmid

   #115996

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
   

   Tags

   HDEL and SP(ER)

   Expression

   Plant

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5FRT/TO-Cezanne-Strep-HA

   Plasmid

   #113492

   Purpose

   Expression of human Cezanne with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Cezanne (OTUD7B Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. H2B-mAvicFP1

   Plasmid

   #129511

   Purpose

   Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   H2B-mAvicFP1
   

   Expression

   Mammalian

   Mutation

   mAvicFP1 fused to the C terminus of human histone…

   Promoter

   CMV

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_RBMX-eA3A-UdgX-nCas9

   Plasmid

   #163546

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p-mCherry-C1-iChR2++

   Plasmid

   #98172

   Purpose

   artificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.

   Depositor

   Peter Hegemann

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct iChR2++ gene
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   A59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V24…

   Promoter

   CMV (+enhancer)

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. WDR5-HaloTag

    Plasmid

    #200881

    Purpose

    Mammalian expression of human WDR5 with C-terminal HaloTag fusion

    Depositor

    Stefan Knapp

    Article

    Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

    Insert

    WDR5-HaloTag (WDR5 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-C9ORF80

    Plasmid

    #128418

    Purpose

    Express C9ORF80 in E. coli with a His tag (C-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    C9ORF80 (INIP Human)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNCST-mAvicFP1

    Plasmid

    #129509

    Purpose

    Expresses mAvicFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    mAvicFP1
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

    Plasmid

    #226350

    Purpose

    Expression of proteasome substrate with tail for molecular ruler

    Depositor

    Andreas Martin

    Article

    Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

    Insert

    titin (TTN Human)
    

    Expression

    Bacterial

    Mutation

    V15P

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221_SLC39A8

    Plasmid

    #132177

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A8 (SLC39A8 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV_hSyn1_AchLightR

    Plasmid

    #187463

    Purpose

    Expresses red acetylcholine indicator AchLightR in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AchLightR
    

    Use

    AAV

    Tags

    Igκ leader

    Promoter

    human Synapsin-1

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKK-TEV-3XFLAG

    Plasmid

    #105784

    Purpose

    Expression of your protein of interest in fusion with 3xFLAG at the C-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-3XFLAG

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNH-TrxT-G-S18

    Plasmid

    #127827

    Purpose

    Production of recombinant human ribosomal protein S18

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS18 (RPS18 Human)
    

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMonAID_dCas9-PmCDA_Hyg_ALS

    Plasmid

    #91692

    Purpose

    Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A and H840A for dead Cas9

    Promoter

    2x35S

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3

    Plasmid

    #170161

    Purpose

    Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME-P2A-H2B-mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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