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MXS_CMV::NeoR-bGHpA

Plasmid

#62437

Purpose

MXS_chaining vector with CMV::NeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Neomycin with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G08

Plasmid

#67142

Purpose

Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His-MYC and mCherry

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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IDP427

Plasmid

#87021

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

SNAP-Nlg1AB-tdMS2(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::PuroR-bGHpA

Plasmid

#62440

Purpose

MXS_chaining vector with PGK::PuroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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mNG-mTq2

Plasmid

#124217

Purpose

Cytoplasmic mNeongreen mTurquoise2 tandem, ~60 % energy transfer. pNM043 - pSAV057-mNG-mTq2

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

mNG-mTq2

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_SIAH2_Sina

Plasmid

#109813

Purpose

Protein expression and purification of SIAH2_Sina

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SIAH2_Sina (SIAH2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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TAL2274

Plasmid

#36711

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MDM2-TALEN_Left (Mdm2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
jk107

Plasmid

#87007

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

SNAP-Nlg1AB-1xλN(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-Calnexin-N-14

Plasmid

#57352

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Calnexin (CANX Human)

Tags

mEos2

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-CAG-EGFP barcode-10-SV40 polyA

Plasmid

#190874

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode10

Use

AAV

Mutation

WT

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHA-ΔN332-Kin1C

Plasmid

#31608

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN332 kinesin-1C (Kif5c Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pAGM7547

Plasmid

#169091

Purpose

MoClo level 1 35S promoter-Db5GT-35S terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-Db5GT-35S terminator

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM7523

Plasmid

#169090

Purpose

MoClo level 1 35S promoter-BvDODA1-Ocs terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvDODA1-Ocs terminator

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM7535

Plasmid

#169089

Purpose

MoClo level 1 35S promoter-BvCYP76AD1-Nos terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvCYP76AD1-Nos terminator

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFPL-Yh2

Plasmid

#61650

Purpose

Gateway vector for generating yellow fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced yellow fluorescent protein

Expression

Plant

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-H1-10

Plasmid

#57383

Purpose

Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

H1 (H1f0 Mouse)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pHH0103_PLAT_Trypsin

Plasmid

#110039

Purpose

Protein expression and purification of PLAT_Trypsin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PLAT_Trypsin (PLAT Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBS/KS-mId2sp

Plasmid

#16044

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id2 (Id2 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 1-195

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
human syndecan 2 EFYA

Plasmid

#64971

Purpose

Constitutive expression in mammalian cells

Depositor

Enric Espel

Article

Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

Insert

syndecan 2 (SDC2 Human)

Expression

Mammalian

Mutation

deleted EFYA

Promoter

CMV

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-Trpa1b-FLAG

Plasmid

#106403

Purpose

Mammalian expression vector containing zebrafish Trpa1b with C-terminal FLAG tag.

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Insert

Trpa1b-FLAG (trpa1b Zebrafish)

Tags

FLAG

Expression

Mammalian

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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human syndecan 2 FAAF

Plasmid

#64972

Purpose

Constitutive expression in mammalian cells

Depositor

Enric Espel

Article

Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

Insert

syndecan 2 (SDC2 Human)

Expression

Mammalian

Mutation

Y179F, S187A, S188A, Y191F

Promoter

CMV

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
pVIM-BC2T

Plasmid

#108967

Purpose

expresses vimentin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

Vimentin

Tags

BC2T

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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Salsa-BC82-P1G10

Plasmid

#202923

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC82

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC82

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_MCS1

Plasmid

#62395

Purpose

MXS_chaining vector with MCS1

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EcoRV-MluI-XhoI-NheI-XbaI-NcoI-KpnI-BclI-BamHI-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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His-Sin3a-115-212(pL01)

Plasmid

#124386

Purpose

E.Coli produced SIN3A PAH1 purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

His-Sin3a-115-212 (SIN3A )

Tags

His

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA7woH

Plasmid

#65020

Purpose

Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, E105K, E108A, D16…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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Salsa-BC2-P1A2

Plasmid

#202843

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC2

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC2

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDS271

Plasmid

#34974

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)

Expression

Yeast

Mutation

T406A, T407A, V416I

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pHH0103_PFN2_Profilin

Plasmid

#110007

Purpose

Protein expression and purification of PFN2_Profilin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PFN2_Profilin (PFN2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-21-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85857

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-21-3p Sensor (MIR21 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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