Addgene

We narrowed to 1,668 results for: GAD

Showing: 1081 - 1120 of 1668 results

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3xnls-G-Ca-FLITS

Plasmid

#191463

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmTurquoise2-N1

Plasmid

#60561

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-C1

Plasmid

#54842

Purpose

Localization: C1 Cloning Vector, Excitation: 434, Emission: 474

Depositor

Michael Davidson, Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Type

Empty backbone

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pmTurquoise2-NES

Plasmid

#36206

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

NES

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-2A-mTurquoise2

Plasmid

#98885

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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mVenus-P115-C1

Plasmid

#112931

Purpose

p115RhoGEF tagged with mVenus for detection of Ga13 activity

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

p115 RhoGEF

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CHOP::GFP

Plasmid

#21898

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

CHOP (Ddit3 Mouse)

Use

Non-viral

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
TurboRFP-pBAD

Plasmid

#54858

Purpose

Localization: Protein Expression Vector , Excitation: 553, Emission: 574

Depositor

Dmitriy Chudakov, Dorus Gadella, Michael Davidson

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Use

Tags

6xHis and TurboRFP

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mKOkappa-2A-mTurquoise2

Plasmid

#98837

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mKOkappa-2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pmVenus-C2-MmMKL2

Plasmid

#67894

Purpose

Expresses a fusion of mVenus with MKL2 (MRTF-B) in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mVenus-MKL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ECFP(W66A)-FRB-MoA

Plasmid

#67904

Purpose

Expresses a Mitochondria located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

ECFP(W66A)-FRB-MoA

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Galphai3-mTurquoise2

Plasmid

#69622

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 3, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai3-mTurquoise2

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pYC3.60-C1

Plasmid

#67899

Purpose

Yellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

YC3.60

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only
Lck-FRB-ECFP(W66A)

Plasmid

#67902

Purpose

Expresses a membrane located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

Lck-FRB-ECFP(W66A)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Galphai1-mTurquoise2

Plasmid

#69620

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 1, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai1-mTurquoise2

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
mKO2-2A-mTurquoise2

Plasmid

#98838

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mKO2-2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FRB-ECFP(W66A)-Giantin

Plasmid

#67903

Purpose

Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

FRB-ECFP(W66A)-Giantin

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Galphai2-mTurquoise2

Plasmid

#69621

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai2-mTurquoise2

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pH2B-SCFP1-2A-mTq2-NES

Plasmid

#60491

Purpose

Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm

Depositor

Dorus Gadella

Article

Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.

Inserts

H2B-SCFP1
mTurquoise2-NES

Use

Tags

SCFP1 and mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
At1R-P2A-mCherry

Plasmid

#112934

Purpose

Plasmid co-producing the AT1R receptor and a mCherry

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

AT1R

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Ga13_2-mTq2

Plasmid

#112933

Purpose

Human Galpha13 tagged with mTurquoise, inserted at residue 128

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

Galpha13

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-C1-pmDH

Plasmid

#67897

Purpose

Expresses a fusion of mCherry with the membrane located DH domain (1-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-pmDH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
G13 sensor (Ga13_2)

Plasmid

#112930

Purpose

A FRET sensor for G13 activation, encoded on a single plasmid.

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

G13 sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LPA2R-P2A-mCherry

Plasmid

#112932

Purpose

Plasmid co-producing the LPA2 receptor and a mCherry

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

LPA2 receptor

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ER-G-Ca-FLITS-KDEL

Plasmid

#191464

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

ER and KDEL

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
9E10.ChWT.Gal4.pCDNA1/AMP

Plasmid

#21914

Depositor

David Ron

Article

Wang et al Science. 1996 May 31. 272(5266):1347-9.

Insert

CHOP (Ddit3 Mouse)

Use

Tags

GAL4-DNA-BD and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-FKBP12-C1-DH

Plasmid

#67901

Purpose

Expresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-FKBP12-DH

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL452

Plasmid

#219502

Purpose

Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL390

Plasmid

#219500

Purpose

Integrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpression

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

Integrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT

Use

Tags

SV40-NLS

Expression

Yeast

Mutation

Promoter

GAL1-GAL10

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCL391

Plasmid

#219501

Purpose

Express Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequence

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

Eco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFR-mScarletI-antiFRET-P2A-EGFP

Plasmid

#191458

Purpose

Compare the intensity of EGFP to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

EGFP

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y

Plasmid

#191459

Purpose

Compare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mTurquoise2_T203Y

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFR-mTq2-antiFRET-P2A-mCherry

Plasmid

#191466

Purpose

Compare the intensity of mCherry to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

mCherry

Use

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFPO-His-G-Ca-FLITS

Plasmid

#191455

Purpose

Bacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

His

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
9E10.ChS78&81A.Gal4.pCDNA1

Plasmid

#21901

Depositor

David Ron

Article

Wang et al Science. 1996 May 31. 272(5266):1347-9.

Insert

CHOP (Ddit3 Mouse)

Use

Tags

GAL4-DNA-BD and Myc

Expression

Mammalian

Mutation

S78A S81A

Promoter

Available since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
dimericTomato-2xrGBD

Plasmid

#176098

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMVdel

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFR-mScarletI-antiFRET-P2A-G-Ca-FLITS

Plasmid

#191457

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) G-Ca-FLITS to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

Insert

G-Ca-FLITS

Use

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG

Plasmid

#129632

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p63RhoGEF619-mVenus-N1

Plasmid

#84339

Purpose

Expresses p63 (619 a.a.) tagged with mVenus

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2016 Nov 11;6:36825. doi: 10.10

Insert

p63RhoGEF (619 aa) (ARHGEF25 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pP2A-mCherry-N1

Plasmid

#84329

Purpose

Encodes P2A-mCherry preceded by a multiple cloning sequence. Enables bicistronic expression of protein of interest and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

P2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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