Addgene

We narrowed to 18,315 results for: Tec

Showing: 1351 - 1400 of 18315 results

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pCX137

Plasmid

#229212

Purpose

CRISPR array plasmid for NOTCH2 (Spacers 1-24)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for NOTCH2 (Spacers 1-24)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pJEC780

Plasmid

#234349

Purpose

Expressed synthetic RNA target for RENDR

Depositor

James Chappell

Article

Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

Insert

Synthetic RNA target for RENDR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

PJ23119

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8r

Plasmid

#208318

Purpose

Overxpression MBP-TadA8r

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r

Use

Tags

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET-PfuX7

Plasmid

#182364

Purpose

Expresses PfuX7 DNA polymerase

Depositor

Morten Norholm

Article

Norholm et al BMC Biotechnol. 2010 Mar 16;10:21. doi:

Insert

PfuX7 DNA polymerase

Use

Tags

His-tag

Expression

Bacterial

Mutation

V93Q

Promoter

T7

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSFV3-lacZ

Plasmid

#92074

Purpose

This is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.

Depositor

Henrik Garoff

Article

Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

Insert

lacZ

Use

Lacz expression plasmid, for run-off transcriptio…

Tags

Expression

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCX459

Plasmid

#229214

Purpose

CRISPR array plasmid for MAP1B (Spacers 1-24)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for MAP1B (Spacers 1-24)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-FRB

Plasmid

#25919

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

Insert

FRB (MTOR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB541

Plasmid

#27394

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

groESL (groL, groS E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2012

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9

Plasmid

#75381

Purpose

dCas9

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

Sp dCas9

Use

Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCX460

Plasmid

#229215

Purpose

CRISPR array plasmid for MAP1B (Spacers 25-48)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for MAP1B (Spacers 25-48)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTR47m4-GFP

Plasmid

#102436

Purpose

Formaldehyde biosensor plasmid with sGFP reporter and modified frm promoter

Depositor

Gregory Stephanopoulos

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

Formaldehyde repressor protein (frmR)
Superfolder GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pNDamMyc

Plasmid

#59217

Purpose

Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.

Depositor

Bas van Steensel

Article

van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

Type

Empty backbone

Use

Damid

Tags

Dam and Myc

Expression

Insect

Mutation

Promoter

Drosophila heat-shock promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pTHSSe_49

Plasmid

#109247

Purpose

pSC101 var7 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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iRFP-C1

Plasmid

#54786

Purpose

Localization: C1 Cloning Vector, Excitation: 690, Emission: 713

Depositor

Vladislav Verkhusha, Michael Davidson

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Type

Empty backbone

Use

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCM157

Plasmid

#45863

Depositor

Mary Lidstrom

Article

Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

Insert

cre recombinase

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8e

Plasmid

#208319

Purpose

Overxpression MBP-TadA8e

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8e

Use

Tags

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
iRFP-N1

Plasmid

#54787

Purpose

Localization: N1 Cloning Vector, Excitation: 690, Emission: 713

Depositor

Vladislav Verkhusha, Michael Davidson

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Type

Empty backbone

Use

Tags

iRFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
multi-shRNA vector

Plasmid

#12391

Depositor

Zuoshang Xu

Article

Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

Insert

mSOD2 shRNA (Sod2 Mouse)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHSSe_42

Plasmid

#109240

Purpose

pSC101 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMG-AIS

Plasmid

#225133

Purpose

For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pH2B_mCherry_IRES_puro2

Plasmid

#21045

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B (H2BC16P Human)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHSSe_45

Plasmid

#109243

Purpose

pSC101 var3 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHSSe_47

Plasmid

#109245

Purpose

pSC101 var5 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHSSe_43

Plasmid

#109241

Purpose

pSC101 var1 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHSSe_44

Plasmid

#109242

Purpose

pSC101 var2 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHSSe_46

Plasmid

#109244

Purpose

pSC101 var4 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHSSe_48

Plasmid

#109246

Purpose

pSC101 var6 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR.hU6hH1

Plasmid

#32686

Purpose

dual shRNA expression cassette entry vector

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6hH1

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pColE1_70a_deGFP_AmpR

Plasmid

#119161

Purpose

Expresses deGFP from a strong sigma 70 promoter

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

deGFP

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB572

Plasmid

#27397

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

ibpA, ibpB (ibpB, ibpA E.coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1C

Plasmid

#139470

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Article

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

stuffer

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTHSSe_50

Plasmid

#109248

Purpose

incW BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PJ23105

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
act5c-LbCas12a

Plasmid

#140620

Purpose

Ubiquitous expression of LbCas12a in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Insert

LbCas12a

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM45244

Plasmid

#219509

Purpose

Transient expression of Antirrhinum majus Rosea1

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

Rosea1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pJOE_pvdJ

Plasmid

#110142

Purpose

Suicide plasmid with repair template for knockout in P. putida

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH21-Tier1-PhEF1a

Plasmid

#169561

Purpose

Tier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_mNeonGreen

Plasmid

#209425

Purpose

Express mNeonGreen in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

mNeonGreen

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pECGMC3

Plasmid

#75441

Purpose

Plasmid for mf-lon mediated mCherry degradation in Lactococcus lactis. Contains mcherry-pdt3

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

mf-lon

Use

Tags

Expression

Bacterial

Mutation

Promoter

nisA promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P12

Plasmid

#125045

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

TSA1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P6

Plasmid

#125039

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

INU1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P5

Plasmid

#125038

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

HSP150 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P18

Plasmid

#125051

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

XYL2 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P14

Plasmid

#125047

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

SSA2 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P13

Plasmid

#125046

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

HSP104 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P17

Plasmid

#125050

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

XYL1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKmK.P9

Plasmid

#125042

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

ALD4 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-20B1(P004)

Plasmid

#184056

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1 preamplifier

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mVenus-N1

Plasmid

#54640

Purpose

Localization: N1 Cloning Vector, Excitation: 515, Emission: 528

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBI/Rabaptin-5

Plasmid

#171851

Purpose

inducible expression of Rabaptin-5 in mammalian cells via co-transfection with pTet-Off.

Depositor

Guangpu Li

Article

Zhu et al PLoS One. 2010 Feb 16;5(2):e9226. doi: 1

Insert

Rabaptin-5 (human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mVenus-C1

Plasmid

#54651

Purpose

Localization: C1 Cloning Vector, Excitation: 515, Emission: 528

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Showing: 1351 - 1400 of 18315 results

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