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We narrowed to 5,876 results for: nls

Showing: 1351 - 1400 of 5876 results

  4. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

    Plasmid
    #107303
    Purpose
    Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    ZFP_VEGFA-TS2
    Use
    CRISPR
    Tags
    2xNES, 3x Flag, 2xNLS, and FRB
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107303-plasmid-map-sequence-id-204639

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  5. pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS

    Plasmid
    #107304
    Purpose
    Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    ZFP_VEGFA-TS3
    Use
    CRISPR
    Tags
    2xNES, 3x Flag, 2xNLS, and FRB
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107304-plasmid-map-sequence-id-204643

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  14. phage - ubc - nls - 2xmcp - mCherry - BirA*

    Plasmid
    #131136
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-mCherry-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131136-plasmid-map-sequence-id-255502

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  15. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI-D10R-E48R
    Use
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60367-plasmid-map-sequence-id-456413

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  16. Col1A2:TFPnls-T2a-mErt-Cre-Ert

    Plasmid
    #111151
    Purpose
    Fibroblasts specific inducible Cre
    Depositor
    Elly Tanaka
    Insert
    TFPnls-T2a-mErt-Cre-Ert
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    wt
    Promoter
    Col1A2
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    111151-plasmid-map-sequence-id-211534

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  17. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

    Plasmid
    #102244
    Purpose
    Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter
    Depositor
    Jacob Corn
    Insert
    dCas9-HA-BFP-KRAB-NLS
    Use
    CRISPR and Lentiviral
    Tags
    HA-TagBFP-KRAB-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102244-plasmid-map-sequence-id-197289

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  23. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Article
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    58477-plasmid-map-sequence-id-90386

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  27. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

    Plasmid
    #192552
    Purpose
    Expresses nuclear localized tdTomato under CAG promoter
    Depositor
    Guoping Feng
    Has Service
    AAV PHP.eB and AAV9
    Insert
    tdTomato
    Use
    AAV
    Tags
    NLS
    Expression
    Mutation
    deleted E273 and G274 compared to FPbase ID: PGG5S
    Promoter
    CAG
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    192552-plasmid-map-sequence-id-403193

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  31. pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

    Plasmid
    #164614
    Purpose
    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.
    Depositor
    Jeffrey Chao
    Article
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Insert
    stdMCP-stdHalo-RH1
    Use
    Lentiviral
    Tags
    HA tag and NLS
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    UbiC
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164614-plasmid-map-sequence-id-325003

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  32. pHR myc-MBP-SspB-iRFP-NLS

    Plasmid
    #223286
    Purpose
    An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.
    Depositor
    Jared Toettcher
    Article
    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860
    Insert
    Myc-MBP-SspB-irFP-NLS
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    223286-plasmid-map-sequence-id-442283

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  33. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

    Plasmid
    #87803
    Purpose
    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    NLS
    PTS1
    Use
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter, tetracycline operator
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87803-plasmid-map-sequence-id-175010

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  34. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid
    #181744
    Purpose
    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY-HF1
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…
    Promoter
    T7
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    181744-plasmid-map-sequence-id-373171

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  44. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Charles Gersbach
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69545-plasmid-map-sequence-id-125605

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  45. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51085-plasmid-map-sequence-id-148706

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  46. pEG302 22aa SunTag NtDRMcd (noNLS) nog

    Plasmid
    #119554
    Purpose
    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)
    Depositor
    Steven Jacobsen
    Article
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    NOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    119554-plasmid-map-sequence-id-238815

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  47. AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51086
    Purpose
    Forebrain principle neuron specific expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa 1.3 kb
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    51086-plasmid-map-sequence-id-469612

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  50. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51084
    Purpose
    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51084-plasmid-map-sequence-id-172966

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Showing: 1351 - 1400 of 5876 results