We narrowed to 1,479 results for: pbluescript
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Plasmid#139209PurposeExpresses activated Fyn kinase in mec-4 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationY531FPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Met133I, M1381I, M186I ELL2
Plasmid#127263PurposeFor in vitro translation of human ELL2 with Met133I, M1381I, M186IDepositorInsertELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationThree Mets (133, 138, 186) to IleuPromoterT7Available sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Sph ELL2
Plasmid#127270PurposeFor in vitro translation of human WT ELL2 with internal HA tagDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationWT Mets and HA tag at SphI site (8th Met)PromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
ELL2mRNA+polyA
Plasmid#127256PurposeFor in vitro translation of human ELL2 with 30 nt pA tailDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationshortens 3'UTR to 40 bp adds A30 tailPromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Xba ELL2
Plasmid#127268PurposeFor in vitro translation of WT ELL2 with HA tag inserted near N-termDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationELL2mRNA+polyA with HA tag inserted at aa12 betwe…PromoterT7Available sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
rat brain inositol 1,4,5-trisphosphate 3-kinase A
Plasmid#122155Purposerat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)DepositorInsertinositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Comp AlphaPS3
Plasmid#14071DepositorInsertDrosphila Comp Alpha PS3 Integrin (scb Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
integrin beta6 777T pBS
Plasmid#13581DepositorInsertintegrin beta 6 777T (ITGB6 Human)
UseTagsExpressionBacterialMutation777T. Lacks last 11 amino acids of cytoplasmic do…PromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEABR NA 3C FLAG SA
Plasmid#234987PurposeFor production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surfaceDepositorInsertEABR-NA
UseTagsHRV 3C site, FLAG, Strep-Tactin (SA)ExpressionMammalianMutationIn the Streptavidin coding sequence Glu44Val/Ser4…PromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS CMV NA 3C FLAG SA
Plasmid#234993PurposeExpression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG proteinDepositorInsertNA
UseTagsHRV 3C site, FLAG, Strep-Tactin (SA)ExpressionMammalianMutationIn the Streptavidin coding sequence Glu44Val/Ser4…PromoterAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNA 3C myc scfvFLAG
Plasmid#234990PurposeExpression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteinDepositorInsertNA-3C-myc-scfvFLAG
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNA 3C myc scfvHA
Plasmid#234991PurposeExpression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteinsDepositorInsertNA-3C-myc-scfvHA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
LB322 (Bcl-2 from ATG to -3934)
Plasmid#15381DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from ATG to -3934 with both P1 and…PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
PG13-luc (wt p53 binding sites)
Plasmid#16442PurposeLuciferase reporter containing 13 copies of the p53-binding consensus sequenceDepositorInsertp53-binding site (TP53 )
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
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INCTbiosyn-pUB-HspINTphiC31
Plasmid#127518PurposePlasmid encodes H. sapiens codon optimized Integrase phiC31.DepositorInsertIntegrase phiC31 coding sequence codon optimized for H. sapiens expression.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMC2-mAC donor (Hygro)
Plasmid#140648PurposeSMC2 tagging with mAID-CloverDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK321 (TOP2A-mAC (Neo))
Plasmid#140652PurposeTOP2A tagging with mAID-CloverDepositorInsertTOP2A (TOP2A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRepair-SGFP2-CTNNB1
Plasmid#153430PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSGFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pInt-ERCreER
Plasmid#190040PurposeThis is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).DepositorInsertERT2-Cre-ERT2 flanked by AAVS1
UseCRISPR and TALENTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSK+ chickBMPR-1B CA
Plasmid#49528Purposecloning vector for constitutively active chick BMP receptor 1BDepositorInsertBMPR-1B CA (BMPR1B Chicken)
UseTagsExpressionBacterialMutationconstitutively active mutation (Q203D); also P2A,…PromoterT3Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-δWT-nAchR
Plasmid#97188PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertDelta nicotinic acetylcholine receptor (Chrnd Mouse)
UseTagsExpressionBacterialMutationWTPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMVpl-γWT-nAchR
Plasmid#97187PurposeTo determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill CoDepositorInsertGamma nicotinic acetylcholine receptor (Chrng Mouse)
UseTagsExpressionBacterialMutationWTPromoterT7Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG_iPE-N
Plasmid#214734Purposeencodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoterDepositorInsertCAG_iPE-N_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG_iPE-C
Plasmid#214735Purposeencodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoterDepositorInsertCAG_iPE-C_bGH
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDiCre-mCherry
Plasmid#164572PurposeIntegration of a DiCre cassette in PbGFP parasite genomeDepositorInsertsFKBP12-CRE59
FRB-CRE60
mCherry
Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
UseCre/LoxTagsExpressionMutationPromoterBidirectional PbEF1alpha promoter (PBANKA_1133300…Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINTBxb1
Plasmid#127519PurposePlasmid encodes H. sapiens codon optimized Integrase Bxb1.DepositorInsertIntegrase Bxb1 coding sequence codon optimized for H. sapiens expression.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
aP2-GFPtpz
Plasmid#114211PurposeaP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophageDepositorInsertaP2 promoter + pOB4 + eGFPtopaz (Fabp4 Aequorea victoria, Mouse, Synthetic)
UseTagsNoneExpressionMammalianMutationPromoteraP2/Fabp4Available sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-PdTK-CAG-mCh-[uBglII]
Plasmid#107280Purposepuro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherryDepositorInsertAAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
UseTagsExpressionMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK322 (TOP2A-mAC (Hygro))
Plasmid#140653PurposeTOP2A tagging with mAID-CloverDepositorInsertTOP2A (TOP2A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-ACTB-mEGFP
Plasmid#115917Purposedonor to insert EGFP at the ACTB locus in human cellsDepositorInsertACTB-mEGFP
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mScI-CTNNB1
Plasmid#153431PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-iDepositorInsertCTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmScarlet-iExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-GAPDH-IRES-EGFP
Plasmid#115918Purposedonor to insert IRES-EGFP at the GAPDH locus in human cellsDepositorInsertIRES-EGFP
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-TOMM20-mEGFP
Plasmid#115923Purposedonor to insert EGFP at the TOMM20 locus in human cellsDepositorInsertTOMM20-mEGFP
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
tet H-ras val12
Plasmid#8900DepositorInsertras val12 (HRAS Human)
UseTagsTet-op promoterExpressionMammalianMutationG12VPromoterAvailable sinceJan. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINTphiC31
Plasmid#127535PurposePlasmid encodes A. thaliana codon optimized Integrase phiC31.DepositorInsertIntegrase phiC31 coding sequence codon optimized for A. thaliana expression.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
LB335 (Bcl-2 from ATG to -1281)
Plasmid#15382DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from ATG to -1281 with P2PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
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INCTbiosyn-pAct-AtINTBxb1
Plasmid#127536PurposePlasmid encodes A. thaliana codon optimized Integrase Bxb1.DepositorInsertIntegrase Bxb1 coding sequence codon optimized for A. thaliana expression.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSK+ mBMPR-1B DN
Plasmid#49530Purposecloning vector for dominant negative mouse BMP receptor 1BDepositorInsertBMPR-1B DN (Bmpr1b Mouse)
UseTagsExpressionBacterialMutationdominant negative mutation (K231R); also L2VPromoterT3Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRepair-SYFP2-CTNNB1
Plasmid#153432PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2DepositorInsertCTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSYFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
DICER KO vector
Plasmid#16656DepositorInsertDicer knock-out (DICER1 Human)
UseAdeno-associated viralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-SEC61B-mEGFP
Plasmid#115921Purposedonor to insert mEGFP at the SEC61B locus in human cellsDepositorInsertSEC61B-mEGFP
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS/U6/RNAi gamma-tubulin shRNA mouse
Plasmid#70666PurposeReduced the expression of gamma-tubulin in murine cellsDepositorInsertUseU6 promoterTagsExpressionMammalianMutationPromoterU6Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-GAPDH-IRES-dTomato
Plasmid#115919Purposedonor to insert IRES-dTomato at the GAPDH locus in human cellsDepositorInsertIRES-dTomato
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
b2AR->M71-IRES-tauGFP-ACNF TV
Plasmid#105198Purposetargeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.DepositorUseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMADM-alpha
Plasmid#36890DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, deleted…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-ONL-CP
Plasmid#65716PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-YNL-CP
Plasmid#65714PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only