We narrowed to 1,297 results for: AP2

1. SVA-lncRNA AK057321 shRNA 1A scramble control

   Plasmid

   #202835

   Purpose

   Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1A

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   pLVX-SVA-lncRNA shRNA 1A scramble control
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TFORF3525

   Plasmid

   #145001

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   THAP2 (THAP2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEBG MKK3b(Glu, delete LXL)

   Plasmid

   #50452

   Purpose

   activates p38 in the signaling pathway

   Depositor

   Roger Davis

   Article

   Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

   Insert

   MKK3b (MAP2K3 Human)
   

   Use

   Tags

   Gst

   Expression

   Mammalian

   Mutation

   deleted L25-I27, changed Serine 218 to Glutamic A…

   Promoter

   SV40

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mcherry

   Plasmid

   #202818

   Purpose

   Lentiviral vector to express 5'-aptamer tagged SVA-lncRNA AK057321 with CMV for mcherry expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   SVA-lncRNA AK057321
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   addition of a dual aptamer RNA tag 5' to the…

   Promoter

   U6

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry

   Plasmid

   #202822

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs that delete exon 1 within the ZNF91 gene
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 - two

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-4T-1 3xHA-MKK4-EE

   Plasmid

   #47577

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MKK4-EE (MAP2K4 Human)
   

   Use

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Lacks the first 36 residues of the RefSeq protein…

   Promoter

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pWZLblasti-HA-MEK1 C121S

   Plasmid

   #53165

   Purpose

   Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C121S

   Promoter

   Available Since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. FLAG-MED30

   Plasmid

   #15426

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED30 (Med30 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pGEX6P1-HA-MEK1 C121S

   Plasmid

   #53164

   Purpose

   Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   C121S

   Promoter

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pSUPER-retro-puro-tetO-Mek1-shRNA

    Plasmid

    #53179

    Purpose

    Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (Map2k1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pGEX6P1-HA-MEK1 CBM

    Plasmid

    #53163

    Purpose

    Expresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    M187A/H188A/M230A/H239A

    Promoter

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pICH86966::AtU6p::sgRNA:NbCysP6

    Plasmid

    #223217

    Purpose

    Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

    Depositor

    Tsepo Tsekoa

    Article

    Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

    Insert

    NbCysP6 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    None

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6 promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-luciferase-reporter-AGPAT3-SVA

    Plasmid

    #202827

    Purpose

    pGL3 luciferase reporter with AGPAT3 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    intronic SVA located in AGPAT3 gene
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCRISPRv2-sgRNAs-del-ZNF91-BFP

    Plasmid

    #202823

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and BFP expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs (each with a U6 promoter) to delete exon 1 within ZNF91 gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 - two

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-EFalpha-SVA-lncRNA-AK057321-mcherry-puro

    Plasmid

    #202800

    Purpose

    Lentiviral vector to express SVA-lncRNA AK057321 with ires for mcherry and puro expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    SVA-lncRNA AK057321
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFalpha (with intron)

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IC65 pEGFPN1 anti-IRSp53 nanobody

    Plasmid

    #135227

    Purpose

    Mammalian expression of anti-IRSp53 (Human) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-IRSp53 (Human) recombinant llama nanobody IC65 (BAIAP2 L. glama (llama))
    

    Use

    Tags

    6xHis-HA-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

    Plasmid

    #170240

    Purpose

    Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S218D, S222D

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL2-Mcl-1 mut

    Plasmid

    #19133

    Depositor

    Wafik El-Deiry

    Article

    Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

    Insert

    Mcl-1 promoter (MCL1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    NFkB binding-site mutant, the sequence GGGGTCTTCC…

    Promoter

    Available Since

    Oct. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEN_TT MEK5-DD-mRFP1

    Plasmid

    #47547

    Purpose

    Gateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

    Insert

    MEK5-DD (MAP2K5 Human)
    

    Use

    Entry vector

    Tags

    mRFP

    Expression

    Mutation

    S311D, T315D

    Promoter

    Available Since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

20. AT4G37250 X020_pECIA2

    Plasmid

    #115019

    Purpose

    Bait vector AT4G37250 X020_pECIA2 should be used with prey vector AT4G37250 X020_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT4G37250 (AT4G37250 Mustard Weed)
    

    Use

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge