We narrowed to 4,185 results for: Gca

1. mGFP-hNET-R121A/K334A/R440A

   Plasmid

   #193366

   Purpose

   expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

   Depositor

   Harald Sitte

   Article

   Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

   Insert

   human Norepinephrine Transporter (SLC6A2 Human)
   

   Use

   Tags

   monomeric GFP (mGFP)

   Expression

   Mammalian

   Mutation

   R121A (CGG to GCG) K334A (AAA to GCA) R440A (CGA …

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFloxin-IRES-Ofd1-Myc-A80T

   Plasmid

   #24575

   Depositor

   Jeremy Reiter

   Article

   Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

   Insert

   Ofd1 (Ofd1 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Myc

   Expression

   Mutation

   Alanine 80 changed to threonine (codon GCA to ACA)

   Promoter

   Available since

   May 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRES2-eGFP-Ofd1-Myc-A80T

   Plasmid

   #24579

   Depositor

   Jeremy Reiter

   Article

   Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

   Insert

   Ofd1 (Ofd1 Mouse)
   

   Use

   Tags

   Myc and eGFP

   Expression

   Mammalian

   Mutation

   Alanine 80 changed to threonine (codon GCA to ACA)

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-T11HA-hMOV10-CD

   Plasmid

   #178908

   Purpose

   expression of catalytic dead hMOV10 K530A

   Depositor

   Constance Ciaudo

   Article

   Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

   Insert

   cDNA Catalytic dead hMOV10 (MOV10 Human)
   

   Use

   Tags

   T11 beta strand of GFP-HA

   Expression

   Mammalian

   Mutation

   K530A catalytic dead AAG>GCA

   Promoter

   CMV protmoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1-scrCdh2.1 GFP

   Plasmid

   #209100

   Purpose

   GFP expressing scramble of shRNA targeting Cdh2

   Depositor

   Cagla Eroglu

   Article

   Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

   Insert

   scrCdh2.1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MYMH 351

   Plasmid

   #138058

   Purpose

   Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

   Depositor

   Meng How Tan

   Article

   Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

   Insert

   pSEVA-331Bb-10AG 11AC-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   AAA GAG GAG GCA

   Promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MYMH 336

   Plasmid

   #138057

   Purpose

   Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

   Depositor

   Meng How Tan

   Article

   Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

   Insert

   pSEVA-331Bb-1AG 2AC 11AC-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GCA GAG GAG ACA

   Promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MYMH 327

   Plasmid

   #138054

   Purpose

   Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

   Depositor

   Meng How Tan

   Article

   Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

   Insert

   pSEVA-331Bb-1AG 2AC-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GCA GAG GAG AAA

   Promoter

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAFMW-Nbr[K187A]

   Plasmid

   #188593

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   K187A [AAA=> GCA]

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmStrawberry-Atg4BC74A

    Plasmid

    #21076

    Depositor

    Tamotsu Yoshimori

    Article

    Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

    Insert

    Atg4B(C74A) (Atg4b Mouse)
    

    Use

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    220-222 TGC(Cys) is changed to GCA (Ala)

    Promoter

    Available since

    June 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. TNPO1-shRNA

    Plasmid

    #86082

    Purpose

    A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Transportin-1 (TNPO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAFMW-Nbr[R280A,K284A]

    Plasmid

    #188598

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R280A [CGA=>GCA], K284A [AAG=> GCG]

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFlare9A-Clip2E9 Mutant

    Plasmid

    #209575

    Purpose

    minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Clip2 (Clip2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…

    Promoter

    CMV

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Pmyo-3::hpo-30 gDNA (pBAB204)

    Plasmid

    #117385

    Purpose

    Allows for body-wall muscle expression of HPO-30

    Depositor

    Kavita Babu

    Article

    Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

    Insert

    genomic DNA of hpo-30 (833 bp)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRA03

    Plasmid

    #103141

    Purpose

    Triple gRNA expression separated with 28nt Csy4 motif

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    gRNAs
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-mCherry-Satb2 sesRNA-2a-msFlag-2a-tTA-WPRE

    Plasmid

    #239028

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse Satb2 (Satb2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUDP044

    Plasmid

    #101168

    Purpose

    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.4_CEACAM6-His

    Plasmid

    #149337

    Purpose

    for eukaryotic expression and purification of CEACAM6-His6

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Use

    Tags

    IL-2 signal peptide and polyhistidine

    Expression

    Mammalian

    Mutation

    point mutation (GGA to GCA) to replace 'G-g…

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSUPER-hHSF2 shRNA

    Plasmid

    #118346

    Purpose

    This plasmid expresses shRNA against hHSF2.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF2 shRNA (HSF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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