We narrowed to 4,253 results for: Gca

1. AiP15580 - pAAV-AiE0780m-minBG-axon-jGCaMP8s-WPRE3-BGHpA (Alias: CN5580)

   Plasmid

   #224202

   Purpose

   AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Insert

   jGCaMP8s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

   Plasmid

   #172917

   Purpose

   LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7b-T2A-tdTomato
   

   Use

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

   Plasmid

   #172920

   Purpose

   LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7s-T2A-tdTomato
   

   Use

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

   Plasmid

   #172919

   Purpose

   LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7f-T2A-tdTomato
   

   Use

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

   Plasmid

   #63885

   Purpose

   An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   ER-localized low-affinity GCaMP3(10.19)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC814 - pAAV-SYN1-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

   Plasmid

   #63887

   Purpose

   An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the SYN1 promoter.

   Depositor

   Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   ER-localized low-affinity GCaMP3(10.19)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human SYN1

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-SARS-CoV-2 Spike-deltaC18 S477N (AAC) T478K (AAA) E484A (GCA)

   Plasmid

   #212472

   Purpose

   Encodes SARS-CoV-2 spike S477N, T478K, E484A for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike S477N, T478K, E484A (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S477N, T478K, E484A

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCL758

   Plasmid

   #219504

   Purpose

   Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mGFP-hNET-R121A/K334A/R440A

   Plasmid

   #193366

   Purpose

   expression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

   Depositor

   Harald Sitte

   Article

   Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

   Insert

   human Norepinephrine Transporter (SLC6A2 Human)
   

   Use

   Tags

   monomeric GFP (mGFP)

   Expression

   Mammalian

   Mutation

   R121A (CGG to GCG) K334A (AAA to GCA) R440A (CGA …

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFloxin-IRES-Ofd1-Myc-A80T

    Plasmid

    #24575

    Depositor

    Jeremy Reiter

    Article

    Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

    Insert

    Ofd1 (Ofd1 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Myc

    Expression

    Mutation

    Alanine 80 changed to threonine (codon GCA to ACA)

    Promoter

    Available since

    May 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIRES2-eGFP-Ofd1-Myc-A80T

    Plasmid

    #24579

    Depositor

    Jeremy Reiter

    Article

    Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

    Insert

    Ofd1 (Ofd1 Mouse)
    

    Use

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Mutation

    Alanine 80 changed to threonine (codon GCA to ACA)

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-T11HA-hMOV10-CD

    Plasmid

    #178908

    Purpose

    expression of catalytic dead hMOV10 K530A

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    cDNA Catalytic dead hMOV10 (MOV10 Human)
    

    Use

    Tags

    T11 beta strand of GFP-HA

    Expression

    Mammalian

    Mutation

    K530A catalytic dead AAG>GCA

    Promoter

    CMV protmoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-scrCdh2.1 GFP

    Plasmid

    #209100

    Purpose

    GFP expressing scramble of shRNA targeting Cdh2

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    scrCdh2.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MYMH 351

    Plasmid

    #138058

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-10AG 11AC-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AAA GAG GAG GCA

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MYMH 336

    Plasmid

    #138057

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-1AG 2AC 11AC-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GCA GAG GAG ACA

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MYMH 327

    Plasmid

    #138054

    Purpose

    Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

    Depositor

    Meng How Tan

    Article

    Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

    Insert

    pSEVA-331Bb-1AG 2AC-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    GCA GAG GAG AAA

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAFMW-Nbr[K187A]

    Plasmid

    #188593

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K187A [AAA=> GCA]

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pmStrawberry-Atg4BC74A

    Plasmid

    #21076

    Depositor

    Tamotsu Yoshimori

    Article

    Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

    Insert

    Atg4B(C74A) (Atg4b Mouse)
    

    Use

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    220-222 TGC(Cys) is changed to GCA (Ala)

    Promoter

    Available since

    June 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TNPO1-shRNA

    Plasmid

    #86082

    Purpose

    A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Transportin-1 (TNPO1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAFMW-Nbr[R280A,K284A]

    Plasmid

    #188598

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    R280A [CGA=>GCA], K284A [AAG=> GCG]

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge