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We narrowed to 24,865 results for: Nes

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Showing: 541 - 560 of 24865 results

  2. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid
    #182652
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available Since
    May 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182652-plasmid-map-sequence-id-440860

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  5. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

    Plasmid
    #127353
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and mVenus
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127353-plasmid-map-sequence-id-246311

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  8. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

    Plasmid
    #127355
    Purpose
    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, NES, V5, and mVenus
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127355-plasmid-map-sequence-id-246258

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  13. AAV-CAG-FLEX-NES-jGCaMP8s-WPRE

    Plasmid
    #186046
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8s under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8s
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186046-plasmid-map-sequence-id-370170

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  14. AAV-CAG-FLEX-NES-jGCaMP8m-WPRE

    Plasmid
    #186045
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8m under the CAG promoter, Cre-dependent expression
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8m
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186045-plasmid-map-sequence-id-370173

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  18. 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

    Plasmid
    #60492
    Purpose
    Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm
    Depositor
    Dorus Gadella
    Article
    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.
    Inserts
    3xnls-SGFP2(T65G)
    SGFP2-3xNES
    Tags
    SGFP2 and SGFP2(T65G)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60492-plasmid-map-sequence-id-96974

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  19. pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE

    Plasmid
    #141236
    Purpose
    AAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)
    Depositor
    Guohong Cui
    Article
    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do
    Insert
    NES-jRGECO1a
    Use
    AAV and Cre/Lox; Cre-off
    Tags
    6xHIS tag and nuclear export signal
    Promoter
    human elongation factor-1 alpha (EF-1 alpha)
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    141236-plasmid-map-sequence-id-432423

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Showing: 541 - 560 of 24865 results