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Plasmid#182385PurposeGives high editing efficiency in barley.DepositorInsertttHsCas12a
UseCRISPR; Coding sequence onlyTagsExpressionMutationD156RPromoterNoneAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJYDC2
Plasmid#162459PurposeYeast display plasmid for expression of C-terminal fusions. Modification of pJYDC1 vector with ALFA tag in MCS.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA-tag, HA tag, HDEL sequence, and eUnaG2 fluor…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_NAP1L1-Halo
Plasmid#175342PurposeProduction of HaloTag fusion proteinDepositorInsertNAP1L1 (NAP1L1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC-PelB-NH10T-AVITAG-GG
Plasmid#215793PurposeCloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-HIS6-GST-TEV-AVITAG-GG
Plasmid#215809PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pH6HTC_NASP-Halo
Plasmid#175340PurposeProduction of HaloTag fusion proteinDepositorInsertNASP (NASP Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-AAVS1-sg
Plasmid#194716PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hAAVS1 locusDepositorArticleInsertAsCpf1 (AAVS1 Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
LZ3 iFE
Plasmid#190143PurposeExpresses LZ3Cas9-POLB (LZ3 iFE)DepositorInsertLZ3Cas9-POLB (LZ3_iFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA
Plasmid#194727PurposeCAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1.DepositorArticleInsertAsCpf1(D908A) (AAVS1 Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
lvl0 mKate2 CDS
Plasmid#153394PurposeFar red fluorescent protein with SV40 NLS, codon optimised for Arabidopsis thalianaDepositorInsertmKate2 CDS
UseTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 dFE
Plasmid#190137PurposeExpresses SpCas9-T4pol (dFE)DepositorInsertSpCas9-T4pol (dFE)
UseCRISPRTags3xFLAGExpressionMammalianMutationCodon Optimized for mammalian cellsPromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH024
Plasmid#186636PurposeaadA.co between homology arms for pilADepositorArticleInsertaadA.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA
Plasmid#194723Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1DepositorArticleInsertAsCpf1 (AAVS1 Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLXMA L2A
Plasmid#153360PurposeExpression vector for plants with medium-copy number origin of replicationDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_RAN-Halo
Plasmid#175339PurposeProduction of HaloTag fusion proteinDepositorInsertRAN (RAN Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB253
Plasmid#170036PurposeEncodes LacI-controlled expression of surface-displayed K5L7 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
K5L7
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceMay 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLXMA _RK2 L1A
Plasmid#153348PurposeExpression vector for plants with low-copy number origin of replicationDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mNeonGreen-G418
Plasmid#236484PurposePlasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on G418.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJethlpAmScarletKm
Plasmid#206820PurposeTemplate for hlpA-mScarlet-I amplification associated with kanamycin resistance geneDepositorInserthlpA
UseSynthetic BiologyTagsmScarletExpressionMutationPromoterAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJethlpAmNeonGreenSpc
Plasmid#206817PurposeTemplate for hlpA-mneongreen amplification associated with spectinomycin resistance geneDepositorInserthlpA
UseSynthetic BiologyTagsmNeonGreenExpressionMutationPromoterAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only