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Beta-2-adrenergic receptor-CFP

Plasmid

#55794

Purpose

Beta-2-adrenergic receptor tagged with ECFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-ECFP (ADRB2 Aequorea victoria, Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-mRFP

Plasmid

#67145

Purpose

Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-mRFP (ADRB2 Human, Discosoma sp.)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-YFP

Plasmid

#67146

Purpose

Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-mCerulean

Plasmid

#66957

Purpose

Beta-2-adrenergic receptor tagged with mCerulean on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-mCerulean (ADRB2 Aequorea victoria, Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 IL2

Plasmid

#67053

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL2 (IL2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 IL6

Plasmid

#67061

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL6 (IL6 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 SMAD2

Plasmid

#67095

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SMAD2 (SMAD2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCx43-7-miRFP670-2-N1

Plasmid

#197223

Purpose

Expresses the fusion protein of Cx43-7-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRFP670-2-Actin-C-18

Plasmid

#197218

Purpose

Expresses the fusion protein of miRFP670-2-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP670-2-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-26a-2

Plasmid

#64230

Purpose

Expresses miR-26a-2 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-26a-2 (MIR26A2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pFB MBD2a-2-strep (pc1794)

Plasmid

#211715

Purpose

Expresses fl. MBD2 2xStrep tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Use

Tags

2xStrep

Expression

Insect

Mutation

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-Nanobody (LaM-2)

Plasmid

#162276

Purpose

Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

mCherry-Nanobody (LaM-2)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 N-2Strep

Plasmid

#210376

Purpose

Expresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-COV-2 N (N SARS-CoV-2)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - Amplicon, JAK2 sgRNA 2

Plasmid

#70660

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-eNme2-T.2-ABE8e

Plasmid

#185669

Purpose

Expresses eNme2-T.2-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-ATG13 (2-197)

Plasmid

#188075

Purpose

To make ATG101/ATG13 HORMA complex for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG13 (ATG13 Human)

Use

Tags

strep-strep-Flag tag

Expression

Mammalian

Mutation

ATG13 (2-197)

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk3_#2-puro

Plasmid

#231982

Purpose

Knockout mouse Ripk3

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-multi-CRISPR-sgMlkl_#2-puro

Plasmid

#231980

Purpose

Knockout mouse Mlkl

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Mlkl Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-multi-CRISPR-sgCasp8_#2-puro

Plasmid

#231978

Purpose

Knockout mouse Casp8

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-multi-CRISPR-sgRipk1_#2-puro

Plasmid

#231983

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge