We narrowed to 882 results for: nanobodies
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Plasmid#132697PurposepDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curveDepositorInsertSb_loop
UseM13 phagmid vectorPromoterlacAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-2xMARS-nGFP-puro
Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-EGFP-P2A-APEX2-NS
Plasmid#108895PurposeMultisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertEGFP-P2A-APEX2
UseMultisite gatewayAvailable SinceMay 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_cys_his
Plasmid#210403PurposeFor expression of VHH with C-terminal cysteine and his tagDepositorTypeEmpty backboneTagscys-his6ExpressionBacterialPromoterlacAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_sortase_his
Plasmid#210406PurposeFor expression of VHH with C-terminal sortase and his tagsDepositorTypeEmpty backboneTagssortase-his6ExpressionBacterialPromoterlacAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his_myc
Plasmid#210526PurposeFor expression of VHH with C-terminal his and myc tagsDepositorTypeEmpty backboneTagsHis6-mycExpressionBacterialPromoterlacAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYTSG
Plasmid#159419PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiaeDepositorInsertthermostable GFP (TGP)
Tagspolyhistidine (poly-His)ExpressionYeastAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb2E7
Plasmid#184681PurposeNIR-Fb to HIV gp41DepositorInsertNIR-Fb2E7
ExpressionMammalianPromoterCMVAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTSG
Plasmid#162389PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cellDepositorInsertthermostable GFP (TGP)
TagsHisExpressionInsectAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb4EIG
Plasmid#184683PurposeNIR-Fb to E.coli dihydrofolate reductaseDepositorInsertNIR-Fb4EIG
ExpressionMammalianPromoterCMVAvailable SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Gly42TAG
Plasmid#125265PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Gly42 nanobody
TagsHis-tagExpressionBacterialPromotertrc promoterAvailable SinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-ppiB
Plasmid#202482PurposeExpresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletionDepositorInsertpeptidyl-prolyl cis-trans isomerase (ppiB)
ExpressionBacterialPromoteryibDpAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-GFPuv
Plasmid#202463PurposeExpresses GFPuv with T7 inductionDepositorInsertGFPuv
ExpressionBacterialPromoterT7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-LCAmp-trxB
Plasmid#202465PurposeExpresses a guide RNA to silence trxB after phosphate depletionDepositorInserttrxB guide RNA
ExpressionBacterialPromoterugpBAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(1-157)
Plasmid#220743PurposeGAL4-sfGFP(1-157) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(1-157)
ExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(158-238)
Plasmid#220744PurposeGAL4-sfGFP(158-238) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(158-238)
ExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-NIR-FbLAG30
Plasmid#220746PurposeGAL4-NIR-FbLAG30 construct expression in mammlian cells.DepositorInsertGAL4-NIR-FbLAG30
UseAffinity Reagent/ AntibodyExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG16
Plasmid#220739PurposeNIR-FbLAG16 expression in mammalian cells.DepositorInsertNIR-FbLAG16
UseAffinity Reagent/ AntibodyExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb(718)
Plasmid#220742PurposeNIR-Fb(718) expression in mammalian cells.DepositorInsertNIR-Fb(718)
UseAffinity Reagent/ AntibodyExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-N1 GBP (GBP-mEos2)
Plasmid#162876PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization MicroscopyDepositorInsertGFP Binding Protein tagged with mEos2
TagsmEos2ExpressionMammalianAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only