We narrowed to 4,373 results for: p2a

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pCMV-PEmax-P2A-BSD

Plasmid

#174821

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

Plasmid

#67979

Purpose

Cas9 activity reporter (control) with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0-Magneto2.0-p2A-mCherry

Plasmid

#74308

Purpose

Bicistronic expression of Magneto2.0 and mCherry in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)

Tags

Flag and p2A-mCherry

Expression

Mammalian

Mutation

TRPV4: delta 760–871

Promoter

CMV

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLX307 P2A GFP Luciferase

Plasmid

#193674

Purpose

Constitutive lentiviral expression of Luciferase

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

Luciferase

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-ME mCherry-P2A-NTRv2-pA

Plasmid

#200532

Purpose

mini circle middle entry plasmid for mCherry- P2A- ntr version 2- pA with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-P2A-BSD

Plasmid

#174819

Purpose

Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PE2-P2A-BSD

Tags

P2A-BSD and SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGs-NLS-TIR1_P2A_NES-TIR1

Plasmid

#117699

Purpose

Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Inserts

TRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1

Tags

HA tag, Myc-NES, and NLS

Expression

Mammalian

Mutation

codon optimized for murine expression

Promoter

None, 2A peptide from porcine teschovirus-1 polyp…

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IDO1-p2a-eGFP

Plasmid

#187026

Purpose

Expresses IDO1 and GFP (seperately) in mammalian cells

Depositor

Geert van den Bogaart

Article

Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

Insert

Indoleamine 2,3-dioxygenase 1 (IDO1 Human)

Tags

P2A-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-CB-FLPe-P2A-tdTomato

Plasmid

#72259

Purpose

Express FLPe and tdTomato under CB promoter

Depositor

Kazuhiro Oka

Insert

FLPe-P2A-tdTomato

Use

Lentiviral

Expression

Mammalian

Mutation

T230I in tdTomato (please see depositor's co…

Promoter

CB promoter and chicken beta-actin + CMV enhancer

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMK432 (mAID-P2A-Hygro)

Plasmid

#168227

Purpose

C-terminal tagging with mAID

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-P2A-Hygro

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMK433 (mAID-P2A-BSD)

Plasmid

#168228

Purpose

C-terminal tagging with mAID

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-P2A-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT_superDn29-dCas9-P2A-GFP

Plasmid

#247166

Purpose

In vitro transcription of human codon optimized fusion of superDn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

superDn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Mutation

M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-BE4GamRA-P2A-Puro

Plasmid

#112673

Purpose

Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4GamRA

Use

Lentiviral

Tags

FLAG

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:NICD-P2A-mRFP

Plasmid

#127594

Purpose

Zebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Insert

NICD (notch1a Zebrafish)

Use

Zebrafish expression

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-OMMjRCaMP-P2A-mito4xGCaMP6f

Plasmid

#127872

Purpose

Dual expression vector for mitochondrial matrix and outer membrane Ca2+ sensors

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Outer membrane localized jRCaMP1b - P2A - mito4xGCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry-p2A-eNpHR3.0-TS

Plasmid

#158703

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

mCherry-p2A-eNpHR3.0-TS

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-4A-P2A-mCherry-NLS

Plasmid

#251133

Purpose

Gachapin-A with a transfection marker mCherry-NLS

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-4A

Tags

P2A-mCherry-NLS

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-4B-P2A-EBFP2-NLS

Plasmid

#251134

Purpose

Gachapin-B with a transfection marker EBFP2-NLS

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-4B

Tags

P2A-EBFP2-NLS

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pANAP-P2A-eRF(E55D)

Plasmid

#241290

Purpose

Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factor

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

the amber suppressor tRNACUA-Leu

Expression

Mammalian

Mutation

mutant E.coli leucyl synthetase specific for Anap…

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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