We narrowed to 18,759 results for: nls
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Ir25a_GAL80
Plasmid#83259PurposeExpresses GAL80 under the control of Ir25a enhancerDepositorInsertIr25a enhancer
ExpressionInsectAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE4
Plasmid#79443Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, BASTADepositorAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE76
Plasmid#80578Purposerecipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptIIDepositorInsertspnos:nptII-tmos
p2x35S:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI
UseCRISPRExpressionPlantMutationD10AAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
TET1CD-CRY2-EGFP
Plasmid#82555PurposeEncodes TET1 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
DNMT3ACD-CRY2-EGFP
Plasmid#82556PurposeEncodes DMNT3 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-eSpCas9-H840A
Plasmid#80454Purposeenhanced SpCas9 mammalian expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertehSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianPromoterCBhAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP244
Plasmid#78978PurposePromoter: ppTEF1, minimal Promoter: GAP. Figure 4. Expresses GFP.DepositorInsertGFP
ExpressionYeastAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35s:dCas:VP64:tNos (GB1189)
Plasmid#75403PurposeTranscriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional ActivatorDepositorInsertdCas:VP64
UseCRISPR and Synthetic BiologyExpressionPlantPromoter35SAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
EZH2-TALEN_Right_No_FLAG
Plasmid#79900PurposeUntagged EZH2-TALEN_Right to target the N-terminus of human EZH2DepositorInsertEZH2-TALEN_Right_No_FLAG (EZH2 Human)
UseTALENAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 Y101A, Y103A
Plasmid#74605Purposeexpresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dyneinDepositorInsertfull length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianMutationY101A, Y103APromoterSFFVAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75244PurposeCRISPR/Cas9 plasmid against human HeliosDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA against human Helios (IKZF2 Human)
UseCRISPRAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST BmOr2
Plasmid#73926PurposeUAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNADepositorInsertBmOr2
ExpressionYeastAvailable sinceMay 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA200
Plasmid#74248PurposepGPD1-dPSTRy(4,3)DepositorInsertpGPD1-dPSTRy(4,3)
ExpressionBacterial and YeastAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA125
Plasmid#74249PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
ExpressionBacterial and YeastPromoterpSTL1Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-LIC1 G domain
Plasmid#74602Purposeexpresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
UseLentiviralTagsHA tagExpressionMammalianPromoterSFFVAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST attB Orco (Y478A)
Plasmid#73928PurposeUAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid changeDepositorAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB2alpha2 35s:hCas9:tNos (GB0639)
Plasmid#68222PurposeTranscriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoterDepositorInserthCas9
UseCRISPR and Synthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM
Plasmid#71811PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
ExpressionInsectAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_HHEX_1
Plasmid#72354PurposeEncodes gRNA for 3' target of human HHEX along with Cas9 with 2A GFPDepositorInsertHHEX (HHEX Human)
UseCRISPRAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-mitf
Plasmid#69801PurposeUsed to make mitf knockout porcine cell lineDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsmitf-sgRNA
SpCas9
ExpressionMammalianPromoterChicken Beta-Actin and U6Available sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pVITRO1-SS-127
Plasmid#68733PurposeZF5-TF with mCherry markerDepositorInsertsZF5-VP64
mCherry
TagsNLS and flagExpressionMammalianAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW121-SS-278
Plasmid#68766PurposeCIS-V2 with N-FLAG and C-HISDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCIS-V2
TagsHIS, NLS, and flagExpressionMammalianAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ158
Plasmid#69307PurposeGateway entry vector with hSpCas9 (Human codon optimized)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9 (Human codon optimized)
UseCRISPRTags3XFLAGMutationNLS fusionAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR29
Plasmid#69255Purposepelt-2::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0, tbb-2 UTR is in wrong o…Promoterelt-2Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
phumanCas9 (GB0575)
Plasmid#68206PurposeProvides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid partDepositorInsertCas9 coding region (human codon optimised)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removed; human codon optimis…Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-271
Plasmid#68772PurposeSensor pair 1 V3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsC-Terminal Sensor 1 V3
N-Terminal Sensor 1 V3
TagsNLS, flag, and nlsExpressionMammalianAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-148
Plasmid#68749PurposeZF8/ZF7-TF with mCherry markerDepositorInsertsZF8/ZF7-VP64
mCherry
TagsNLS and flagExpressionMammalianAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-149
Plasmid#68750PurposeZF6/ZF10-TF with mCherry markerDepositorInsertsZF6/ZF10-VP64
mCherry
TagsNLS and flagExpressionMammalianAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW121-SS-276
Plasmid#68764PurposeCIS-V1 with N-FLAG and C-HISDepositorInsertCIS-V1
TagsHIS, NLS, and flagExpressionMammalianAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW121-SS-277
Plasmid#68765PurposeCIS-V1m with N-FLAG and C-HISDepositorInsertCIS-V1m
TagsHIS, NLS, and flagExpressionMammalianAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
px330_SMAD exon 9 gRNA
Plasmid#68352Purposepx330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSMAD exon 9 gRNA
ExpressionMammalianPromoterU6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
px330_SMAD exon 2 gRNA
Plasmid#68350Purposepx330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSMAD exon 2 gRNA
ExpressionMammalianPromoterU6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-Emi1_control UTRs
Plasmid#67925PurposeExpresses mCherry-Emi1 with control UTRs in mammalian cellsDepositorAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX398 Nitratifractor salsuginis str DSM 16511 Cas9
Plasmid#68334PurposeNitratifractor salsuginis str DSM 16511 Cas9DepositorInsertNsCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable sinceAug. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX381 Filifactor alocis Cas9
Plasmid#68318PurposeFilifactor alocis Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFaCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX395 Roseburia intestinalis Cas9
Plasmid#68332PurposeRoseburia intestinalis Cas9DepositorInsertRiCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only
