We narrowed to 64,925 results for: Cel

1. V1-MESA-45F-Tev

   Plasmid

   #84501

   Purpose

   MESA protease chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, and Tev protease

   Depositor

   Joshua Leonard

   Article

   Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

   Insert

   V1-MESA-45F-Tev
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSR49

   Plasmid

   #69259

   Purpose

   phlh-8::tagBFP2

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Insert

   TagBFP2
   

   Use

   Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0, TagBFP2 contains 2 sing…

   Promoter

   hlh-8

   Available since

   Oct. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. GG-EBNA-OCT4

   Plasmid

   #69537

   Purpose

   Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   5 concatenated gRNA transcriptional cassettes targeting OCT4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-sgRNA(MS2)-puro-barcode

   Plasmid

   #89493

   Purpose

   Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   sgRNA (with MS2 loop)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. V2-MESA-35F-M-tTA

   Plasmid

   #84502

   Purpose

   MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and tTA

   Depositor

   Joshua Leonard

   Article

   Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

   Insert

   V2-MESA-35F-M-tTA
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK-flag-USP19 C506S

   Plasmid

   #78584

   Purpose

   Expression flag-USP19 C506S in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 C506S (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   mutate 506 cysteine residue to serine

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

7. HUW-TetO-hNanog

   Plasmid

   #25700

   Depositor

   Rudolf Jaenisch

   Article

   Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

   Insert

   Nanog (NANOG Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pBS-KS-attB2-SA(1)-T2A-Gal4

   Plasmid

   #62900

   Purpose

   Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-KS-attB2-SA(2)-T2A-Gal4

   Plasmid

   #62901

   Purpose

   Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR_PGK_ALPPL2 synNotch

    Plasmid

    #188376

    Purpose

    Lentiviral vector - constitutive expression of an anti-ALPPL2 synNotch with a Gal4VP64 transcriptional factor

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    PGK_antiALPPL2_synNotch_Gal4VP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-ML1N*2

    Plasmid

    #67678

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of ML1N*2

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    ML1N*2
    

    Use

    Gateway multisite 3' entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. HS-PCNA-GFP

    Plasmid

    #105942

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pME-APEX2-NS

    Plasmid

    #67669

    Purpose

    Gateway entry clone containing APEX2 with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2
    

    Use

    Gateway multisite middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ef1a_NANOG_Ires_Puro

    Plasmid

    #18920

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    NANOG (NANOG Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

15. V1-MESA-45F-M-tTA

    Plasmid

    #84500

    Purpose

    MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and tTA

    Depositor

    Joshua Leonard

    Article

    Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

    Insert

    V1-MESA-45F-M-tTA
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-LifeAct-Venus

    Plasmid

    #87613

    Purpose

    Actin-targeted Venus (YFP).

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-Venus
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK-flag-USP19 1-1290

    Plasmid

    #78579

    Purpose

    Expression flag-USP19 1-1290 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 1-1290 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 1291 residue to stop condon

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

18. USP19 sgRNA2

    Plasmid

    #78586

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA2 (USP19 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

19. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2

    Plasmid

    #79902

    Purpose

    Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Insert

    3xFLAG_Twin_Strep_EZH2
    

    Use

    CRISPR and TALEN

    Tags

    3xFLAG_Twin_Strep

    Expression

    Mammalian

    Mutation

    Promoter

    PGK1

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENeGFP PCNAL2 pc653

    Plasmid

    #167564

    Purpose

    The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

    Depositor

    Cristina Cardoso

    Article

    Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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