Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

177,587 results

Showing: 2811 - 2820 of 177587 results

  5. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65767-plasmid-map-sequence-id-471631

    Enlarge

  8. pAC152-dual-dCas9VP64-sgExpression

    Plasmid
    #48238
    Purpose
    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    HA Tag, HA-Tag, and VP64
    Expression
    Mammalian
    Mutation
    D10A;H840A
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    48238-plasmid-map-sequence-id-69545

    Enlarge

  10. pUC57 APEX

    Plasmid
    #40306
    Purpose
    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pea APEX (Synthetic)
    Use
    Unspecified
    Mutation
    K14D, W41F, E112K (relative to wild-type)
    Available since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    40306-plasmid-map-sequence-id-115990

    Enlarge

Showing: 2811 - 2820 of 177587 results