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pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

Plasmid

#154303

Purpose

Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Inserts

histone H1
γ-tubulin

Use

Dictyostelium expression

Tags

mEGFP and mRuby3

Promoter

actin15

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7b2

Plasmid

#14623

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b2 (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pOCC175

Plasmid

#118890

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6-MBP, cleavable with 3C protease; mGFP, cleav…

Expression

Insect

Promoter

polH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMMP-puro-Flag-HA-NR6A1

Plasmid

#46833

Depositor

Phil Sharp

Article

Gurtan et al Genes Dev. 2013 Apr 15;27(8):941-54. doi

Insert

NR6A1 (Nr6a1 Mouse)

Use

Retroviral

Tags

Flag-HA, plus 8-amino acid residue linker

Expression

Mammalian

Mutation

None

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only
DNMT1

Plasmid

#25165

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

dnmt1.0351.0600 (DNMT1 Human)

Tags

His

Expression

Bacterial

Available Since

April 4, 2016

Availability

Industry, Academic Institutions, and Nonprofits
pBBR6k-J23109-GFPuv

Plasmid

#106392

Purpose

pBBR6k-GFPuv with Anderson promoter J23109 expressing lacI

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

LlacO-1

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7g1

Plasmid

#14624

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7g1 (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
Olig001

Plasmid

#170716

Purpose

Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

Depositor

Thomas McCown

Article

Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

Insert

Olig001 capsid gene / rep 2

Use

AAV

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMCB306-GFP-mito

Plasmid

#229232

Purpose

Expresses GFP localized to mitochondria

Depositor

Suzanne Pfeffer

Article

Bagnoli et al Proc Natl Acad Sci U S A. 2025 Feb 4;122

Insert

EGFP-MAOA (MAOA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-tetR-T2A-tSapphire

Plasmid

#176312

Purpose

construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

Depositor

Irmela Jeremias

Article

Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

Inserts

tetR
T-sapphire

Use

Lentiviral

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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