4,183 results

1. pAAVS1-NC-CRISPRi (Gen3)

   Plasmid

   #73499

   Purpose

   Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Beta-arrestin2 GFP WT

   Plasmid

   #35411

   Depositor

   Robert Lefkowitz

   Article

   Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

   Insert

   βarrestin2 (Arrb2 Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2CT-His-MBP-Lbu_C2c2_WT

   Plasmid

   #83482

   Purpose

   Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_WT
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIS0

   Plasmid

   #12178

   Purpose

   3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

   Depositor

   David Bartel

   Article

   Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cdc2-HA

   Plasmid

   #1888

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   Cdc2 (CDK1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. TetO-FUW-cMYC

   Plasmid

   #20324

   Depositor

   Rudolf Jaenisch

   Article

   Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

   Insert

   cMYC (Myc Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. TAp63alpha-FLAG

   Plasmid

   #27008

   Depositor

   David Sidransky

   Insert

   TAp63alpha (TP63 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCGN N-Ras wt

   Plasmid

   #14723

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

   Insert

   N-ras (NRAS Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

   Plasmid

   #37120

   Purpose

   DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   TdTomato-EGFP
   

   Use

   AAV and Cre/Lox; Cre-switch

   Promoter

   EF1a

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti PGK Blast V5-LUC (w528-1)

    Plasmid

    #19166

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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