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pCMV-GFP-ILVBLpA-G10C-C21G

Plasmid

#120207

Purpose

Human ILVBL poly(A) site reporter, stem loop mutant rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

ILVBL poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-TFE3pA-G9C-C19G

Plasmid

#120203

Purpose

Human TFE3 poly(A) site reporter, stem loop mutant rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1-CENPB

Plasmid

#120208

Purpose

gRNA targeting the stem loop of CENPB poly(A) site

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pWT016g

Plasmid

#96849

Purpose

processed HHR-sgRNA-GFP

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

processed HHR-sgRNA-GFP

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Sgd1

Plasmid

#100000

Purpose

Shuttle Sgd1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Sgd1 (SGD1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Bms1

Plasmid

#100001

Purpose

Shuttle Bms1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Bms1 (BMS1 Budding Yeast)

Expression

Yeast

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb1

Plasmid

#99981

Purpose

Shuttle yeast Ckb1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb1 (CKB1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Ckb2

Plasmid

#99982

Purpose

Shuttle yeast Ckb2 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Ckb2 (CKB2 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Utp11

Plasmid

#99984

Purpose

Shuttle yeast Utp11 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Utp11 (UTP11 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Pfa1

Plasmid

#99999

Purpose

Shuttle yeast Pfa1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Pfa1 (SQS1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-Cka1

Plasmid

#99979

Purpose

Shuttle yeast Cka1 into Gateway destination vector

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Cka1 (CKA1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221 Tma16

Plasmid

#63779

Purpose

yeast ORF Tma16 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Tma16 (TMA16 Budding Yeast)

Use

Gateway donor vector

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMES140

Plasmid

#71849

Purpose

Complementation of NME1 gene disruption or mutation in yeast

Depositor

David Clayton, Mark Schmitt

Article

Schmitt et al Mol Cell Biol. 1993 Dec;13(12):7935-41.

Insert

NME1 (NME1 Budding Yeast)

Expression

Yeast

Promoter

NME1

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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CJ64 (miR-1003 5' splice site mut)

Plasmid

#17785

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene 5' splice mut

Use

Drosophila p-element vector

Mutation

5' splice site mut + compsensatory stem

Available Since

May 12, 2008

Availability

Academic Institutions and Nonprofits only
CJ26 (miR-1003 3'ssAG->GA)

Plasmid

#17765

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene 3'ssAG->GA

Use

Drosophila metallothionein gene promoter drives e…

Expression

Insect

Mutation

splice site mutation

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pDEST-CMV-N-EGFP

Plasmid

#122842

Purpose

N-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pB-mTSPAN4-GFP

Plasmid

#210329

Purpose

Overexpression of mTSPAN4

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

mTSPAN4 (Tspan4 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-N-mCherry

Plasmid

#123215

Purpose

N-terminal mCherry tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-pSu9-HaloTag7-mGFP

Plasmid

#184905

Purpose

Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

Presequence of Fo-ATPase subunit 9

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3

Plasmid

#184901

Purpose

Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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