We narrowed to 35,304 results for: lat
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Plasmid#102992PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsPromoterU6Available SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only
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pSF4 CMV intron renilla TRICK CTE polyA
Plasmid#84443PurposeTRICK reporter mRNADepositorInsertRenill-TRICK
UseLuciferase; FrtExpressionMammalianPromoterTet-CMVAvailable SinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_30bpLinker
Plasmid#157789PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_30bp linker
ExpressionBacterialAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJA327
Plasmid#74486Purposesuperfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)DepositorInsertsuperfolder GFP
ExpressionWormPromotermyo-3Available SinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCOM4
Plasmid#49798PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-LRF
Plasmid#66936PurposeDestination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).DepositorTypeEmpty backboneUseLuciferaseExpressionMammalianPromoterSV-40Available SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFP
Plasmid#28011DepositorInsertCFP
ExpressionBacterialAvailable SinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 NSP16_nostop
Plasmid#149318PurposeGateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertNSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable SinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcU6_3 MS2 sgRNA
Plasmid#92394PurposeChick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCPDepositorInsertchick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
UseCRISPRExpressionMammalianPromoterchick U6.3Available SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-o-YFP
Plasmid#55776PurposeThis G protein alpha-o construct contains internal insertions of YFP and the EE epitopeDepositorInsertG protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
TagsEE epitope was introduced internally with D167E a…ExpressionMammalianMutationA BglII site was removed from the 5' untrans…PromoterCMVAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-DBD-LBD
Plasmid#171237PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)DepositorInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-NTD-DBD
Plasmid#171236PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)DepositorInsert2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-117:POLR2A-mEGFP
Plasmid#164500PurposeHomology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2ADepositorInsertPOLR2A Homology Arms with mEGFP-linker (POLR2A Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceFeb. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSpdCas9-huTET1CD-T2A-GFP (PX458)
Plasmid#129025Purposetargeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNADepositorInsertdCas9-huTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-EGFPExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW-miR-124-GFP-Puro
Plasmid#117321PurposeOverexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intronDepositorInserthsa-miR-124-1 (MIR124-1 Human)
UseLentiviralTagsGFP-PuroExpressionMammalianPromoterEF1aAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
LMP AMPK alpha1 hp1
Plasmid#89489PurposeshRNA against AMPK alpha1 in mouse/human to knock down protein expressionDepositorUseMouse Targeting and RetroviralExpressionMammalianPromoterU6 (The GFP-Puro is from a PGK promoter; the shRN…Available SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-DNER
Plasmid#51053Purposeexpression of HA-DNER in mammalian cellsDepositorInsertDNER (Dner Mouse)
TagsHA and signal peptideExpressionMammalianMutationwithout endogenous signal peptide (1-221bp)PromoterCMVAvailable SinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR_Pax6-RE_tBFP_PGK_mCitrine
Plasmid#188388PurposeLentiviral vector - tBFP reporter for Pax6-based SNIPRs with a constitutive mCitrineDepositorInsertPax6-RE_tBFP_PGK_mCitrine
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5-79
Plasmid#118087PurposeN-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmidDepositorInsertN-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid
UseSynthetic BiologyAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSII403
Plasmid#35436DepositorTypeEmpty backboneUseYeast integrating vectorPromoterNoneAvailable SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only