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pcDNA3.1-HA-mUbp43

Plasmid

#12454

Depositor

Dong-Er Zhang

Article

Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

Insert

UBP43 (Usp18 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

Plasmid

#74309

Purpose

Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells

Depositor

Ali Guler

Article

Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

Insert

TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Rat, Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMOS003: GCaMPer calcium sensor (endoplasmic reticulum)

Plasmid

#163047

Purpose

GCaMPer calcium sensor (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

GCaMPer

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-ProRS-His

Plasmid

#124117

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Prolyl-tRNA ligase (proS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBlu/gRNA

Plasmid

#59188

Purpose

Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Guide RNA Cassette

Use

CRISPR

Expression

Plant

Promoter

U6 (Arabidopis)

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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FOP-GFP.mC

Plasmid

#35492

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xFOP-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

mutated 7xTCF/LEF

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID pRetrox

Plasmid

#155203

Purpose

For doxycycline-inducible expression of 3xHA-TurboID on N-terminus of bait protein

Depositor

Kyle Roux

Article

May et al Cells. 2020 Apr 25;9(5). pii: cells90510

Insert

3xHA-TurboID

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Promoter

tight TRE promotor

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBRPB CAG-mCherry-IP

Plasmid

#106333

Purpose

PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

mCherry-IRES-Puro

Use

Piggybac transposon

Expression

Mammalian

Promoter

CAG

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CoChR-GFP

Plasmid

#59070

Purpose

AAV-mediated expression of CoChR-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CoChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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Pax5

Plasmid

#35003

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

Paired box protein Pax-5 (Pax5 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pBAD33-mf-lon

Plasmid

#21867

Depositor

Robert Sauer

Article

Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

Lon (Mfl404 Mesoplasma florum)

Expression

Bacterial

Mutation

all TGA codons changed to TGG codons

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pCMMP-MCS-IRES-mRFP

Plasmid

#36972

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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Desmoglein2-mCherry

Plasmid

#36991

Depositor

Kathleen Green

Article

Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

Insert

Dsg2 (DSG2 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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pNLS-TetR-GFP

Plasmid

#103838

Purpose

Flourescent marker for tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

NLS-TetR (tetR )

Tags

EGFP

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pJBEI-6411

Plasmid

#47050

Purpose

BglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coli

Depositor

Taek Soon Lee

Article

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

ahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase

Use

Synthetic Biology; Bglbrick

Expression

Bacterial

Promoter

Pbad

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pEAK-Flag/INPP4B

Plasmid

#24324

Depositor

Lewis Cantley

Article

Gewinner et al Cancer Cell. 2009 Aug 4. 16(2):115-25.

Insert

Inositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

none

Available Since

May 11, 2010

Availability

Academic Institutions and Nonprofits only

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pDsRed Monomer C1-YAP2-S127A mutant

Plasmid

#19058

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

Red-FPP

Expression

Mammalian

Mutation

Serine 127 changed to Alanine

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCS2-Flag-TTGZFP-FokI-DD

Plasmid

#18755

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

Insert

zinc finger nuclease

Expression

Bacterial

Mutation

RNA expression vector for expressing the zinc fin…

Available Since

July 11, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-2NLS-Cas9-6XMS2

Plasmid

#166033

Purpose

Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

Depositor

Yujia Cai

Article

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Insert

SpCas9

Use

CRISPR and Lentiviral

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AktAR

Plasmid

#118462

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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