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pXD71Cas10.0-Pct5.1-crRNA(AarI)

Plasmid

#191633

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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p221z-erTagRFP

Plasmid

#71266

Purpose

Entry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

TagRFP

Use

Gateway Cloning

Tags

ER retention signal HDEL and signal peptide (ER)…

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR223-BCRF1(vIL10)

Plasmid

#195777

Purpose

Gateway Entry Clone of Epstein-Barr virus BCRF1, vIL-10

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BCRF1, vIL-10 (BCRF1 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_EGFP

Plasmid

#25899

Purpose

Gateway entry vector containing EGFP.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

EGFP

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223_Luciferase

Plasmid

#25894

Purpose

Gateway entry vector containing Luciferase.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

Luciferase

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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DT050A

Plasmid

#159411

Purpose

Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23101 promoter

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1777 (pEffector)

Plasmid

#160731

Purpose

Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins and crRNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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gfasPurple chromoprotein

Plasmid

#117849

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, gfasPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_LacZ

Plasmid

#25893

Purpose

Gateway entry vector containing LacZ.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

LacZ

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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aeBlue chromoprotein

Plasmid

#117846

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, aeBlue

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-NEK9W967A

Plasmid

#168271

Purpose

Expresses LIR (LC3-interacting region) mutant NEK9

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 979 to alanine

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4-NHK-MYC-His6

Plasmid

#185350

Purpose

Assessing of ubiquitination in cells

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

a1-antitrypsin Hong Kong Null mutant

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

NHK mutant

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR221-TCF7

Plasmid

#79498

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

TCF7 (TCF7 Human)

Use

Gateway Cloning

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSL3617 (pCRISPR(BbsI)_Pse)

Plasmid

#200902

Purpose

Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseCRISPR (entry)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-AtMIR390a-B/c

Plasmid

#51778

Purpose

Gateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

Depositor

James Carrington

Article

Carbonell et al Plant Physiol. 2014 Mar 19.

Insert

AtMIR390a-B/c

Use

Gateway Cloning

Mutation

A. thaliana MIR390a precursor sequence including …

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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eforRed chromoprotein

Plasmid

#117837

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses eforRed chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, eforRed

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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amajLime chromoprotein

Plasmid

#117843

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amajLime

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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fwYellow chromoprotein

Plasmid

#117841

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, fwYellow

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-Turbo-NES-L2

Plasmid

#127349

Purpose

gateway entry vector for making N-terminal YFP-TurboID fusions with non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, NES, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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