Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,771 results for: NUC

Showing: 5641 - 5660 of 12771 results

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pEPOR0SP0017

Plasmid

#117527

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.0 (no stop codon)

Use

CRISPR

Mutation

K810A, K1003A, R1060A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0015

Plasmid

#117525

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (no stop codon)

Use

CRISPR

Mutation

K855A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0001

Plasmid

#117523

Purpose

Level0 Golden Gate part: CDS, SpCas9-DE (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-DE (no stop codon)

Use

CRISPR

Mutation

D1135E

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0050

Plasmid

#117617

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0049

Plasmid

#117616

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0051

Plasmid

#117618

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_AtMPK19 {AT3G14720}(pEPOR1CB0081)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]

Expression

Plant

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pRS316Nab3-11

Plasmid

#110535

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Mutation

two missense mutations creating Nab3-11

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3-11

Plasmid

#110534

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Mutation

two missense mutations creating Nab3-11

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMAL-SsoPCNA3

Plasmid

#107069

Purpose

Expresses S. solfataricus PCNA3 fused to MBP in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Sci Rep. 2016 May 27;6:26588. doi: 10.10

Insert

S. solfataricus PCNA3

Tags

6xHis-thrombin cleavage site and MBP

Expression

Bacterial

Promoter

tac promoter

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 AAAA

Plasmid

#87600

Purpose

Encodes Prp5 AAAA for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

D136A, P137A, L138A, D139A

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 TAG

Plasmid

#87603

Purpose

Encodes Prp5 SAT motif to TAG for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

S446T, T448G

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 K335R

Plasmid

#87299

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 K335R

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

K335R

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGBKT7-CLF1

Plasmid

#87548

Purpose

Encodes Y2H Gal4 DNA binding domain for Clf1

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

CLF1 (CLF1 Budding Yeast)

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 D450G

Plasmid

#87303

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 D450G

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

D450G

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 P369K

Plasmid

#87302

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 P369K

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

P369K

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 H331Q

Plasmid

#87296

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 H331Q

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

H331Q

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 H331D

Plasmid

#87295

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 H331D

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

H331D

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 N295D

Plasmid

#87294

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 N295D

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

N295D

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 R294L

Plasmid

#87293

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 R294L

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD

Expression

Bacterial and Yeast

Mutation

R294L

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 N295D/D450G

Plasmid

#87287

Purpose

Encodes Hsh155 N295D D450G for expression in yeast

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N295D & D450G

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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Showing: 5641 - 5660 of 12771 results

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