We narrowed to 14,476 results for: nts

1. pUC19-Hph

   Plasmid

   #188741

   Purpose

   pUC19 plasmid (MCS intact) with Hygromycin selection marker

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   Hygromycin
   

   Use

   Filamentous fungal expression

   Promoter

   gpdA

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. N8his-GFPenhancer-GGGGS4-LaG16

   Plasmid

   #140442

   Purpose

   We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

   Depositor

   Motoyuki Hattori

   Article

   Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

   Insert

   GFPenhancer-GGGGS4-LaG16
   

   Tags

   8 histidine tag

   Expression

   Bacterial

   Promoter

   T7 promotor

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAH750

   Plasmid

   #91212

   Purpose

   protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters, structurally optimized gRNA scaffolds)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   2 gRNAs targeting tomato ARF8A
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBR BsmAIM Kan + p15A BsaIR Amp

   Plasmid

   #206208

   Purpose

   Contains two plasmids. Constitutive expression of BsmAIM and inducible expression of BsaIR

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

   Insert

   BsamAIM and BsaIR
   

   Expression

   Bacterial

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA5/FRT/TO_TOMM20_HaloTag7

   Plasmid

   #169330

   Purpose

   Expression of TOMM20-HaloTag7 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag7 (TOMM20 Human)
   

   Tags

   HaloTag7

   Expression

   Mammalian

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAGM65879

   Plasmid

   #153214

   Purpose

   Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGM9037

   Plasmid

   #153225

   Purpose

   Template for PCR amplification of the constant part of guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   guide RNA constant part, carotenoid marker
   

   Use

   Synthetic Biology

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Myc-E-Syt1

   Plasmid

   #66833

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt1 (ESYT1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDualEX-SMN2

   Plasmid

   #214431

   Purpose

   Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN2 EXON 7 (SMN2 Human)
   

   Expression

   Mammalian

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGM65881

    Plasmid

    #153215

    Purpose

    Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-eYFP

    Plasmid

    #104055

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV2, and AAV5

    Insert

    EYFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUSE-Src-YF-UniRapR-mCerulean-myc

    Plasmid

    #45381

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    Src-YF-UniRapR-mCerulean-myc
    

    Tags

    Myc, Myr, and mCerulean

    Expression

    Mammalian

    Mutation

    changed mSrc Tyrosine 529 to Phenylalanine

    Promoter

    CMV

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDualIN-SMN2

    Plasmid

    #214430

    Purpose

    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    SMN2 EXON 7 (SMN2 Human)
    

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDR111_mannose_Bgal NLS

    Plasmid

    #188398

    Purpose

    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Tags

    PhoD secretion peptide and SV40 NLS

    Expression

    Bacterial

    Promoter

    D-mannose

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SlugABE-NNG

    Plasmid

    #214356

    Purpose

    Expresses SlugABE-NNG

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. mH2A1.1-CT-MYC

    Plasmid

    #45166

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Tags

    HIS and MYC

    Expression

    Mammalian

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid

    #89355

    Purpose

    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    G532R/K595R

    Promoter

    CMV

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Pb-TRE3G-SpCas9-IRES-blast

    Plasmid

    #195506

    Purpose

    Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. mH2A1.2-CT-MYC

    Plasmid

    #45168

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Tags

    HIS and MYC

    Expression

    Mammalian

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3 IKKe flag

    Plasmid

    #26201

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon (IKBKE Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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