164,722 results

1. CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

   Plasmid

   #190162

   Purpose

   Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

   Insert

   mcherry_(3'UTR, No Actin)_24Broccoli
   

   Use

   Tags

   24×Broccoli

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCas9-GFP

   Plasmid

   #78546

   Purpose

   Fusion protein Cas9+GFP

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Fusion protein with Cas9

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAT-PB-CD2APtk

   Plasmid

   #86004

   Purpose

   homology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassette

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   DsRedEx_T2A_puro_dTK
   

   Use

   Piggybac exciseable

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Cas9-NLS-6xHis

   Plasmid

   #62933

   Purpose

   Expression of Cas9-NLS-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9-NLS-6xHis
   

   Use

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET31b-T7-Spinach2

   Plasmid

   #79783

   Purpose

   Expresses Spinach2 under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   T7-tRNA-Spinach2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBG100-TCPTP

   Plasmid

   #33365

   Depositor

   Ambra Pozzi

   Article

   Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

   Insert

   TCPTP-45 (PTPN2 Human)
   

   Use

   Tags

   6xHIS - cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pCMV CEPIA3mt

   Plasmid

   #58219

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA3mt
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V E334D M339L

   Promoter

   CMV

   Available Since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138132

   Purpose

   Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   EGFP-anti-mouse-IgG(kappa)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZsGreen1-N1

   Plasmid

   #54702

   Purpose

   Localization: N1 Cloning Vector, Excitation: 493, Emission: 505

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   ZsGreen1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-EGFR-L858R

    Plasmid

    #116276

    Purpose

    Lentiviral expression of EGFR L858R

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L858R

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PP_072_pAAV_hSyn_DIO-LR-Voltron2-P2A-LR-CheRiff-HA

    Plasmid

    #230988

    Purpose

    Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Article

    Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

    Insert

    Cre-dependent, membrane-localized optopatch for neuronal dendrites
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0042-PguCas13b crRNA backbone

    Plasmid

    #103853

    Purpose

    For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-PguCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pwtCas9-bacteria

    Plasmid

    #44250

    Purpose

    aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    wild-type Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available Since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5_mCherry-P300

    Plasmid

    #238258

    Purpose

    For overexpression of mCherry-P300

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P300
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDL025_HA-rb_IgG_HC_15F11

    Plasmid

    #193622

    Purpose

    HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti_HA_15F11 rabbit heavy chain
    

    Use

    Tags

    signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDL026_HA-rb_IgG_LC_15F11

    Plasmid

    #193623

    Purpose

    HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-HA_15F11 rabbit light chain
    

    Use

    Tags

    signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pZR069_CROPseq-sgPTPRC-CRISPRi

    Plasmid

    #180271

    Purpose

    Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    PTPRC (CD45) sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEVOL-pAzF[TAG]

    Plasmid

    #164579

    Purpose

    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Inserts

    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    araBAD, glnS, and proK

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRE-Tn7-163

    Plasmid

    #118575

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sYFP2
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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