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pHH0103_VCL_Vinculin

Plasmid

#110045

Purpose

Protein expression and purification of VCL_Vinculin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VCL_Vinculin (VCL Human)

Expression

Bacterial

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Lifeact-7

Plasmid

#54846

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Lifeact-PAmCherry-miniSOG

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
Dom34+GST

Plasmid

#19099

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Protein DOM34 (DOM34 Budding Yeast)

Tags

C tag, GST, and N tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
LacD.1_WT_pET21a

Plasmid

#29789

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase (lacD.1 S. pyogenes (serotype M1))

Tags

His

Expression

Bacterial

Available Since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
LacD_L165E/L275S_pET21a

Plasmid

#29788

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Mutation

L165E and L275S

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
Tip alpha (21-192)

Plasmid

#23157

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-20

Available Since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
Ta1098

Plasmid

#19072

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pWRKY70::LUC

Plasmid

#141171

Purpose

Luminescent reporter for salicylic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtWRKY70 promoter

Use

Luciferase

Expression

Plant

Promoter

AtWRKY70, AT3G56400

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Clover-CytERM-N-17

Plasmid

#56301

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

CytERM

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pHH0103_TOM1L2_VHS

Plasmid

#109901

Purpose

Protein expression and purification of TOM1L2_VHS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TOM1L2_VHS (TOM1L2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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TAL2292

Plasmid

#36725

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

ALK-TALEN_Left (Alk Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
907K

Plasmid

#160236

Purpose

FlightN-PipR-VP16-Sv40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

pristinamycin repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCALNL-BsaI-EGFP

Plasmid

#123136

Purpose

Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

EGFP, mRFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

cBA

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBT100-neo

Plasmid

#123137

Purpose

Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Neomycin, poly-A terminator

Use

Synthetic Biology; Golden gate donor vector

Promoter

none

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-MP-HRW-CRY2olig-VHH(GFP)

Plasmid

#126026

Purpose

express RFP-tagged LARIAT variant for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHRolig-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

CRY2 E490G

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_PPARD_Hormone-recep

Plasmid

#109955

Purpose

Protein expression and purification of PPARD_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PPARD_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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TAL2380

Plasmid

#36789

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

ABL1-TALEN_Left (Abl1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
mCherry-Supervillin-45

Plasmid

#55136

Purpose

Localization: Membrane/Actin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Supervillin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pOVC1_X_VfAU1-LOV

Plasmid

#131185

Purpose

Expresses VfAU1-LOV domain (Vaucheria frigida) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

VfAU1-LOV

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_VfAU1-LOV_X

Plasmid

#131174

Purpose

Expresses VfAU1-LOV domain (Vaucheria frigida) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

VfAU1-LOV

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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SPDEF_pet28a

Plasmid

#131643

Purpose

Bacterial expression of ETS gene SPDEF

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

SPDEF (SPDEF Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(cAMP-0.4)/pRSETB

Plasmid

#53590

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Tags

6xHis

Expression

Bacterial

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pOVC1_X_MxCarH-CBD

Plasmid

#131189

Purpose

Expresses MxCarH-CBD (Myxococcus xanthus) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

MxCarH-CBD

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_TtCarH-CBD

Plasmid

#131190

Purpose

Expresses TtCarH-CBD (Thermus termophilus) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

TtCarH-CBD

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-210-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85920

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-210-3p Sensor (MIR210 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-p19-T7-GagB500

Plasmid

#46314

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
GagB500 DNA-1
linker
GagB500 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-325-Decoy.hPGK.GFP.WPRE

Plasmid

#46610

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-325 Decoy

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pET20b-CeNLuc-6xHis

Plasmid

#135933

Purpose

Expresses the CeNLuc LumiFluor in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C2-GFP

Plasmid

#46043

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C2 (Myh14 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV immediate early

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only
mRuby2-Myotilin-C-14

Plasmid

#55907

Purpose

Localization: Cytoskeleton, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Myotilin (MYOT Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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